LOCUS       sequence-98974-e         680 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses CibN-dCas9 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 680)
  AUTHORS   Polstein LR, Gersbach CA
  TITLE     A light-inducible CRISPR-Cas9 system for control of endogenous gene 
            activation.
  JOURNAL   Nat Chem Biol. 2015 Feb 9. doi: 10.1038/nchembio.1753.
  PUBMED    25664691
REFERENCE   2  (bases 1 to 680)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2015 Feb 9. doi: 10.1038/nchembio.1753."
COMMENT     Sequence Label: pcDNA3.1-CibN-dCas9
FEATURES             Location/Qualifiers
     source          1..680
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(12..32)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 taggcgcgcc caactttgcg tttctttttc gggctagcct tggatccggc aattgggtca
       61 cctcctagct gactcaaatc aatgcgtgtt tcataaagac cagtgatgga ttgatggata
      121 agagtggcat ctaaaacttc ttttgtagac gtatatcgtt tacgatcaat tgttgtatca
      181 aaatatttaa aagcagcggg agctccaaga ttcgtcaacg taaataaatg aataatattt
      241 tctgcttgtt cacgtattgg tttgtctcta tgtttgttat atgcactaag aactttatct
      301 aaattggcat ctgctaaaat aacacgctta gaaaattcac tgatttgctc aataatctca
      361 tctaaataat gcttatgctg ctccacaaac aattgttttt gttcgttatc ttctggacta
      421 cccttcaact tttcataatg actagctaaa tataaaaaat tcacatattt gcttggcaga
      481 gccagctcat ttcctttttg taattctccg gcactagcca gcatccgttt acgaccgttt
      541 tctaactcaa aaagactata tttaggtagt ttaatgatta agtctttttt aacttcctta
      601 tatcctttag cttctaaaaa gtcaatcgga tttttttcaa aggaacttct ttccataatt
      661 gtgatcccta gtaactcttt
//
