LOCUS       sequence-98978-e         575 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses dCas9-CibN in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 575)
  AUTHORS   Polstein LR, Gersbach CA
  TITLE     A light-inducible CRISPR-Cas9 system for control of endogenous gene 
            activation.
  JOURNAL   Nat Chem Biol. 2015 Feb 9. doi: 10.1038/nchembio.1753.
  PUBMED    25664691
REFERENCE   2  (bases 1 to 575)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2015 Feb 9. doi: 10.1038/nchembio.1753."
COMMENT     Sequence Label: pcDNA3.1-dCas9-CibN
FEATURES             Location/Qualifiers
     source          1..575
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             94..159
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             166..186
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct ggctagcgcc atggactaca aagaccatga cggtgattat
      121 aaagatcatg acatcgatta caaggatgac gatgacaaga tggcccccaa gaagaagagg
      181 aaggtgggcc gcggaatgga taagaaatac tcaataggct tagctatcgg cacaaatagc
      241 gtcggatggg cggtgatcac tgatgaatat aaggttccgt ctaaaaagtt caaggttctg
      301 ggaaatacag accgccacag tatcaaaaaa aatcttatag gggctctttt atttgacagt
      361 ggagagacag cggaagcgac tcgtctcaaa cggacagctc gtagaaggta tacacgtcgg
      421 aagaatcgta tttgttatct acaggagatt ttttcaaatg agatggcgaa agtagatgat
      481 agtttctttc atcgacttga agagtctttt ttggtggaag aagacaagaa gcatgaacgt
      541 catcctattt ttggaaatat agtagatgaa gttgc
//
