LOCUS       sequence-98979-e        8535 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Allows the integration of GFP MPS1 K-R in the genome and 
            Tet-inducible expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8535)
  AUTHORS   Tighe A, Staples O, Taylor S
  TITLE     Mps1 kinase activity restrains anaphase during an unperturbed 
            mitosis and targets Mad2 to kinetochores.
  JOURNAL   J Cell Biol. 2008 Jun 16;181(6):893-901. doi: 10.1083/jcb.200712028.
            Epub 2008 Jun 9.
  PUBMED    18541701
REFERENCE   2  (bases 1 to 8535)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1083/jcb.200712028"; journalName: "J Cell Biol"; date: 
            "2008-06-16- 16"; volume: "181"; issue: "6"; pages: "893-901"
COMMENT     Sequence Label: pcDNA5 FRT TO GFP MPS1 K-R
FEATURES             Location/Qualifiers
     source          1..8535
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    820..838
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    841..859
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     869..893
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(1151..1153,1154..1156,1157..1867)
                     /codon_start=1
                     /product="Emerald GFP"
                     /label=EmGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1196..1217)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1457..1476)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1804..1825
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(4487..4504)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4493..4717
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4850..4869)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    5001..5048
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             5056..6078
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             5056..6078
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    6208..6329
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6245..6264)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6299..6318
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6378..6394)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6378..6394)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6391..6413)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6402..6418
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6426..6432,6433..6450,6451..6456))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6471..6492
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6609..6626)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6780..7368)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6860..6879)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7539..8330,8331..8399))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8162..8181
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8400..8504)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
      841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
      901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
      961 ctctagcgtt taaacttaag cttggtaccc cggggaattc ggcagatccc gaggtccgac
     1021 agcgcccggc ccagatcccc acgcctgcca ggagcaagcc gagagccagc cggccggcgc
     1081 actccgactc cgagcagtct ctgtccttcg acccgagccc cgcgcccttt ccgggacccc
     1141 tgccccgcgg atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt
     1201 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga
     1261 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc
     1321 ctggcccacc ctcgtgacca ccttgaccta cggcgtgcag tgcttcgccc gctaccccga
     1381 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg
     1441 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg
     1501 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat
     1561 cctggggcac aagctggagt acaactacaa cagccacaag gtctatatca ccgccgacaa
     1621 gcagaagaac ggcatcaagg tgaacttcaa gacccgccac aacatcgagg acggcagcgt
     1681 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc
     1741 cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga
     1801 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct
     1861 gtacaagctc gagggatcca tggaatccga ggatttaagt ggcagagaat tgacaattga
     1921 ttccataatg aacaaagtga gagacattaa aaataagttt aaaaatgaag accttactga
     1981 tgaactaagc ttgaataaaa tttctgctga tactacagat aactcgggaa ctgttaacca
     2041 aattatgatg atggcaaaca acccagagga ctggttgagt ttgttgctca aactagagaa
     2101 aaacagtgtt ccgctaagtg atgctctttt aaataaattg attggtcgtt acagtcaagc
     2161 aattgaagcg cttcccccag ataaatatgg ccaaaatgag agttttgcta gaattcaagt
     2221 gagatttgct gaattaaaag ctattcaaga gccagatgat gcacgtgact actttcaaat
     2281 ggccagagca aactgcaaga aatttgcttt tgttcatata tcttttgcac aatttgaact
     2341 gtcacaaggt aatgtcaaaa aaagtaaaca acttcttcaa aaagctgtag aacgtggagc
     2401 agtaccacta gaaatgctgg aaattgccct gcggaattta aacctccaaa aaaagcagct
     2461 gctttcagag gaggaaaaga agaatttatc agcatctacg gtattaactg cccaagaatc
     2521 attttccggt tcacttgggc atttacagaa taggaacaac agttgtgatt ccagaggaca
     2581 gactactaaa gccaggtttt tatatggaga gaacatgcca ccacaagatg cagaaatagg
     2641 ttaccggaat tcattgagac aaactaacaa aactaaacag tcatgcccat ttggaagagt
     2701 cccagttaac cttctaaata gcccagattg tgatgtgaag acagatgatt cagttgtacc
     2761 ttgttttatg aaaagacaaa cctctagatc agaatgccga gatttggttg tgcctggatc
     2821 taaaccaagt ggaaatgatt cctgtgaatt aagaaattta aagtctgttc aaaatagtca
     2881 tttcaaggaa cctctggtgt cagatgaaaa gagttctgaa cttattatta ctgattcaat
     2941 aaccctgaag aataaaacgg aatcaagtct tctagctaaa ttagaagaaa ctaaagagta
     3001 tcaagaacca gaggttccag agagtaacca gaaacagtgg caatctaaga gaaagtcaga
     3061 gtgtattaac cagaatcctg ctgcatcttc aaatcactgg cagattccgg agttagcccg
     3121 aaaagttaat acagagcaga aacataccac ttttgagcaa cctgtctttt cagtttcaaa
     3181 acagtcacca ccaatatcaa catctaaatg gtttgaccca aaatctattt gtaagacacc
     3241 aagcagcaat accttggatg attacatgag ctgttttaga actccagttg taaagaatga
     3301 ctttccacct gcttgtcagt tgtcaacacc ttatggccaa cctgcctgtt tccagcagca
     3361 acagcatcaa atacttgcca ctccacttca aaatttacag gttttagcat cttcttcagc
     3421 aaatgaatgc atttcggtta aaggaagaat ttattccatt ttaaagcaga taggaagtgg
     3481 aggttcaagc aaggtatttc aggtgttaaa tgaaaagaaa cagatatatg ctataaaata
     3541 tgtgaactta gaagaagcag ataaccaaac tcttgatagt taccggaacg aaatagctta
     3601 tttgaataaa ctacaacaac acagtgataa gatcatccga ctttatgatt atgaaatcac
     3661 ggaccagtac atctacatgg taatggagtg tggaaatatt gatcttaata gttggcttaa
     3721 aaagaaaaaa tccattgatc catgggaacg caagagttac tggaaaaata tgttagaggc
     3781 agttcacaca atccatcaac atggcattgt tcacagtgat cttaaaccag ctaactttct
     3841 gatagttgat ggaatgctaa agctaattgc ctttgggatt gcaaaccaaa tgcaaccaga
     3901 tacaacaagt gttgttaaag attctcaggt tggcacagtt aattatatgc caccagaagc
     3961 aatcaaagat atgtcttcct ccagagagaa tgggaaatct aagtcaaaga taagccccaa
     4021 aagtgatgtt tggtccttag gatgtatttt gtactatatg acttacggga aaacaccatt
     4081 tcagcagata attaatcaga tttctaaatt acatgccata attgatccta atcatgaaat
     4141 tgaatttccc gatattccag agaaagatct tcaagatgtg ttaaagtgtt gtttaaaaag
     4201 ggacccaaaa cagaggatat ccattcctga gctcctggct catccatatg ttcaaattca
     4261 aactcatcca gttaaccaaa tggccaaggg aaccactgaa gaaatgaaat atgttctggg
     4321 ccaacttgtt ggtctgaatt ctcctaactc cattttgaaa gctgctaaaa ctttatatga
     4381 acactatagt ggtggtgaaa gtcataattc ttcatcctcc aagacttttg aaaaaaaaag
     4441 gggaaaaaaa tgagcggccg cgggcccgtt taaacccgct gatcagcctc gactgtgcct
     4501 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
     4561 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg
     4621 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac
     4681 aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc
     4741 tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     4801 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     4861 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     4921 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     4981 ggtgatggtt cacgtaccta gaagttccta ttccgaagtt cctattctct agaaagtata
     5041 ggaacttcct tggccaaaaa gcctgaactc accgcgacgt ctgtcgagaa gtttctgatc
     5101 gaaaagttcg acagcgtctc cgacctgatg cagctctcgg agggcgaaga atctcgtgct
     5161 ttcagcttcg atgtaggagg gcgtggatat gtcctgcggg taaatagctg cgccgatggt
     5221 ttctacaaag atcgttatgt ttatcggcac tttgcatcgg ccgcgctccc gattccggaa
     5281 gtgcttgaca ttggggaatt cagcgagagc ctgacctatt gcatctcccg ccgtgcacag
     5341 ggtgtcacgt tgcaagacct gcctgaaacc gaactgcccg ctgttctgca gccggtcgcg
     5401 gaggccatgg atgcgatcgc tgcggccgat cttagccaga cgagcgggtt cggcccattc
     5461 ggaccgcaag gaatcggtca atacactaca tggcgtgatt tcatatgcgc gattgctgat
     5521 ccccatgtgt atcactggca aactgtgatg gacgacaccg tcagtgcgtc cgtcgcgcag
     5581 gctctcgatg agctgatgct ttgggccgag gactgccccg aagtccggca cctcgtgcac
     5641 gcggatttcg gctccaacaa tgtcctgacg gacaatggcc gcataacagc ggtcattgac
     5701 tggagcgagg cgatgttcgg ggattcccaa tacgaggtcg ccaacatctt cttctggagg
     5761 ccgtggttgg cttgtatgga gcagcagacg cgctacttcg agcggaggca tccggagctt
     5821 gcaggatcgc cgcggctccg ggcgtatatg ctccgcattg gtcttgacca actctatcag
     5881 agcttggttg acggcaattt cgatgatgca gcttgggcgc agggtcgatg cgacgcaatc
     5941 gtccgatccg gagccgggac tgtcgggcgt acacaaatcg cccgcagaag cgcggccgtc
     6001 tggaccgatg gctgtgtaga agtactcgcc gatagtggaa accgacgccc cagcactcgt
     6061 ccgagggcaa aggaatagca cgtactacga gatttcgatt ccaccgccgc cttctatgaa
     6121 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat
     6181 ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa
     6241 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     6301 ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag
     6361 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     6421 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     6481 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     6541 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     6601 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     6661 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     6721 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     6781 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     6841 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     6901 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     6961 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     7021 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     7081 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     7141 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     7201 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     7261 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     7321 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     7381 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     7441 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     7501 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     7561 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     7621 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     7681 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     7741 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     7801 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     7861 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     7921 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     7981 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     8041 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     8101 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     8161 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     8221 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     8281 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     8341 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     8401 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     8461 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     8521 gtgccacctg acgtc
//
