LOCUS       sequence-98980-e         627 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses dCas9-CibN in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 627)
  AUTHORS   Polstein LR, Gersbach CA
  TITLE     A light-inducible CRISPR-Cas9 system for control of endogenous gene 
            activation.
  JOURNAL   Nat Chem Biol. 2015 Feb 9. doi: 10.1038/nchembio.1753.
  PUBMED    25664691
REFERENCE   2  (bases 1 to 627)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2015 Feb 9. doi: 10.1038/nchembio.1753."
COMMENT     Sequence Label: pcDNA3.1-dCas9-CibN
FEATURES             Location/Qualifiers
     source          1..627
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(516..536)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tccgttttct ccaattcctt cgtcacttta gatgaatcat tagagaaatt gttctcttct
       61 ttcttggctt tgtgtttcat cttcttgatg ctttttgtgc tcccattgtt ttgctctgtt
      121 attttcgact tctcttcttc tccttcttct actaggtcat ctctgttcat cgtcatcttc
      181 ttcttcttct cattacaatc cttagtctct gtatcaaact tccgtttctt gaaatttcca
      241 gtcccaagcg tcgtttccgg cgaaattgag agtcttgaat caccttccac cgtcgtctca
      301 ccgtacatca tcggaagatt caaaccggca gtcgaaagat agctgtccat ctcgccgccg
      361 gtaatcattg aagaatcggc aaagaagccg gcgagaggag aatcaaaggt gggattgagg
      421 tacttgaggt gagccctttg gcgctctagg accgacatgt caggaaaatt gagcaaaagg
      481 tcacctccta tagctccatt catggtggcg cgcccaactt tgcgtttctt tttcgggcta
      541 gccttggatc cggcaattgg gtcacctcct agctgactca aatcaatgcg tgtttcataa
      601 agaccagtga tggattgatg gataaga
//
