LOCUS       sequence-98982-e         681 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Cry2FL-VP64 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 681)
  AUTHORS   Polstein LR, Gersbach CA
  TITLE     A light-inducible CRISPR-Cas9 system for control of endogenous gene 
            activation.
  JOURNAL   Nat Chem Biol. 2015 Feb 9. doi: 10.1038/nchembio.1753.
  PUBMED    25664691
REFERENCE   2  (bases 1 to 681)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2015 Feb 9. doi: 10.1038/nchembio.1753."
COMMENT     Sequence Label: pcDNA3.1-Cry2FL-VP64
FEATURES             Location/Qualifiers
     source          1..681
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     49..68
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        49..67
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             93..158
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             165..185
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 agctctctgg ctaactagag aacccactgc ttactggctt atcgaaatta atacgactca
       61 ctatagggag acccaagctg gctagcgcca tggactacaa agaccatgac ggtgattata
      121 aagatcatga catcgattac aaggatgacg atgacaagat ggcccccaag aagaagagga
      181 aggtgggccg catgaagatg gacaaaaaga ctatagtttg gtttagaaga gacctaagga
      241 ttgaggataa tcctgcatta gcagcagctg ctcacgaagg atctgttttt cctgtcttca
      301 tttggtgtcc tgaagaagaa ggacagtttt atcctggaag agcttcaaga tggtggatga
      361 aacaatcact tgctcactta tctcaatcct tgaaggctct tggatctgac ctcactttaa
      421 tcaaaaccca caacacgatt tcagcgatct tggattgtat ccgcgttacc ggtgctacaa
      481 aagtcgtctt taaccacctc tatgatcctg tttcgttagt tcgggaccat accgtaaagg
      541 agaagctggt ggaacgtggg atctctgtgc aaagctacaa tggagatcta ttgtatgaac
      601 cgtgggagat atactgcgaa aagggcaaac cttttacgag tttcaattct tactggaaga
      661 aatgcttaga tatgtcgatt g
//
