LOCUS       sequence-98983-e         618 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Cry2FL-VP64 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 618)
  AUTHORS   Polstein LR, Gersbach CA
  TITLE     A light-inducible CRISPR-Cas9 system for control of endogenous gene 
            activation.
  JOURNAL   Nat Chem Biol. 2015 Feb 9. doi: 10.1038/nchembio.1753.
  PUBMED    25664691
REFERENCE   2  (bases 1 to 618)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2015 Feb 9. doi: 10.1038/nchembio.1753."
COMMENT     Sequence Label: pcDNA3.1-Cry2FL-VP64
FEATURES             Location/Qualifiers
     source          1..618
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(27..53)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(60..209)
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             complement(219..239)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ggccgcgaat tcctaccgat tcaagaagcg tagtccggaa cgtcgtacgg gtagttaatt
       61 aacatatcga gatcgaaatc gtccagagca tcggagccga gcatgtccag atcaaagtca
      121 tccaatgcgt cgcttcccaa catatccagg tcaaagtcat cgagggcatc agaacccagc
      181 atgtcgagat cgaaatcgtc cagcgcgtcg gcgcgcccaa ctttgcgttt ctttttcggg
      241 ctagccttgg atccggcaat tggtttgcaa ccattttttc ccaaacttgt agtaatctga
      301 tcagatgaat cttgaatacc ttccagattc ttcccttctg atgccaacga gcaagactgc
      361 gaaacgaaaa acacactcga agaagaagaa gattcagcag tcgaaaacaa ctccctttca
      421 tcgaatcccc tagatttctt catgtctctc tcttcttctt cctcaggttt cactctcttt
      481 gacccgttgt aacgaacagc cgaaggtact tgttggtcat tagaagacac agatgggcaa
      541 agaccaggtt ctttaatggt attagcccct aaggcctcga agctatctgc tacaatctca
      601 tcaggtgctg ctccgatc
//
