LOCUS       sequence-98984-e         469 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses CibN-dCas9-CibN in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 469)
  AUTHORS   Polstein LR, Gersbach CA
  TITLE     A light-inducible CRISPR-Cas9 system for control of endogenous gene 
            activation.
  JOURNAL   Nat Chem Biol. 2015 Feb 9. doi: 10.1038/nchembio.1753.
  PUBMED    25664691
REFERENCE   2  (bases 1 to 469)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2015 Feb 9. doi: 10.1038/nchembio.1753."
COMMENT     Sequence Label: pcDNA3.1-CibN-dCas9-CibN
FEATURES             Location/Qualifiers
     source          1..469
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gcagagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa attaatacga
       61 ctcactatag ggagacccaa gctggctagc gccatggact acaaagacca tgacggtgat
      121 tataaagatc atgacatcga tataaatgga gctataggag gtgacctttt gctcaatttt
      181 cctgacatgt cggtcctaga gcgccaaagg gctcacctca agtacctcaa tcccaccttt
      241 gattctcctc tcgccggctt ctttgccgat tcttcaatga ttaccggcgg cgagatggac
      301 agctatcttt cgactgccgg tttgaatctt ccgatgatgt acggtgagac gacggtggaa
      361 ggtgattcaa gactctcaat ttcgccggaa acgacgcttg ggactggaaa tttcaagaaa
      421 cggaagtttg atacagagac taaggattgt aatgagaaga agaagaaga
//
