LOCUS       sequence-98985-e         964 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Donor plasmid for HR-mediated recombination to insert puromycin 
            resistance and append a destabilization domain to the N-terminus of 
            TCOF1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 964)
  AUTHORS   Park A, Won ST, Pentecost M, Bartkowski W, Lee B
  TITLE     CRISPR/Cas9 allows efficient and complete knock-in of a 
            destabilization domain-tagged essential protein in a human cell 
            line, allowing rapid knockdown of protein function.
  JOURNAL   PLoS One. 2014 Apr 17;9(4):e95101. doi: 
            10.1371/journal.pone.0095101. eCollection 2014.
  PUBMED    24743236
REFERENCE   2  (bases 1 to 964)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-04-17"; volume: "9(4):e95101. doi"; pages: " 
            10.1371/journal.pone.0095101. eCollection 2014"
COMMENT     Sequence Label: PuroR-P2A-DD-TCOF1-donor
FEATURES             Location/Qualifiers
     source          1..964
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     3..22
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3..21
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tgtaatacga ctcactatag ggcgaattgg gccctctaga tgcatgctcg agcggccgcc
       61 agtgtgatgg atatctgcag aattcgccct tttggccggc ccttattgcg gtgctaagca
      121 tatagtcact tttgtgattc tttgatttgt ctaccttact agagtgtaag ctccaggagg
      181 gctgagagat ggtgctcacc attcgaacac caggaagcac cctgagtacc ggattaacag
      241 ttgaattaat caagtaagaa atgactgtac cacgagttcg agtaccaaag ctgaccgtct
      301 cagtacagac aatcgctaaa ggcctgagtt ccagctcaac taagaggctg tagggtccct
      361 tttccaggcg ctcccccgag gggctggtcg caatccaccc ccgagactcg cagcaacctc
      421 cttgccccag ggggggccgc gcagagccta gccacgtggc gggagactta ggaaacttaa
      481 aatacccgct accaggaagg gcgtggaggt aagggaagcg ggaattacaa agttcggccc
      541 ctgcgagcac actttctggc ccaacccact gcccggatga ggaaagtgag gcccagggga
      601 actgaagtcg cagtgggaag gcagagattg cggctcctcc ggtacccctg gccgcgccgt
      661 cgagcactgc gggacccggc tccgggcgcc tgccgggtcg cctggggacg gggcgcgggc
      721 cgcgggccgc tgatctccac atcttgcacg cggccctcgc acacggccca cgaacgctta
      781 cctggccgct ctgctccttc acttcccgcg ccgcacgcac atagccagcc cgcagcagat
      841 ggtggtagat caggggaagt agctcccgcc gcttcctggc ctcggccatt gaaccgccgc
      901 caccagatcc acctccacca gatccacctc caccttccgg ttttagaagc tccacatcga
      961 agac
//
