LOCUS       sequence-98990-e        2799 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A direct fusion of sfGFP to the 24xGCN4_v1 peptide array.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2799)
  AUTHORS   Tanenbaum ME, Gilbert LA, Qi LS, Weissman JS, Vale RD
  TITLE     A Protein-Tagging System for Signal Amplification in Gene Expression
            and Fluorescence Imaging.
  JOURNAL   Cell. 2014 Oct 8. pii: S0092-8674(14)01227-6. doi: 
            10.1016/j.cell.2014.09.039.
  PUBMED    25307933
REFERENCE   2  (bases 1 to 2799)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2014 
            Oct 8. pii: S0092-8674(14)01227-6. doi: 10.1016/j.cell.2014.09.039."
COMMENT     Sequence Label: pcDNA4TO-sfGFP-24xGCN4_v1
FEATURES             Location/Qualifiers
     source          1..2799
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             10..720
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     CDS             10..720
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Nager et al., 2011)"
                     /label=superfolder GFP
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(37..65)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     660..680
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     CDS             748..813
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             829..894
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             910..975
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             991..1056
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             1072..1137
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             1153..1218
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             1234..1299
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             1315..1380
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             1396..1461
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             1477..1542
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             1558..1623
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             1639..1704
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     primer_bind     1720..1742
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     primer_bind     1743..1765
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             1768..1833
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             1849..1914
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             1930..1995
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             2011..2076
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             2092..2157
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             2173..2238
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             2254..2319
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             2335..2400
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             2416..2481
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             2497..2562
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             2578..2643
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             2659..2724
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
ORIGIN
        1 atgagcggta gcaaaggaga agaacttttc actggagttg tcccaattct tgttgaatta
       61 gatggtgatg ttaatgggca caaattttct gtccgtggag agggtgaagg tgatgctaca
      121 aacggaaaac tcacccttaa atttatttgc actactggaa aactacctgt tccgtggcca
      181 acacttgtca ctactctgac ctatggtgtt caatgctttt cccgttatcc ggatcacatg
      241 aaacggcatg actttttcaa gagtgccatg cccgaaggtt atgtacagga acgcactata
      301 tctttcaaag atgacgggac ctacaagacg cgtgctgaag tcaagtttga aggtgatacc
      361 cttgttaatc gtatcgagtt aaagggtatt gattttaaag aagatggaaa cattcttgga
      421 cacaaactcg agtacaactt taactcacac aatgtataca tcacggcaga caaacaaaag
      481 aatggaatca aagctaactt caaaattcgc cacaacgttg aagatggttc cgttcaacta
      541 gcagaccatt atcaacaaaa tactccaatt ggcgatggcc ctgtcctttt accagacaac
      601 cattacctgt cgacacaatc tgtcctttcg aaagatccca acgaaaagcg tgaccacatg
      661 gtccttcttg agtttgtaac tgctgctggg attacacatg gcatggatga gctctacaaa
      721 ggtcccggtc cgggaggttc tggaggattg ttgcccaaaa actaccactt ggagaacgag
      781 gtcgcaaggt tgaaaaaatt ggtcggtgag cgtggcggct caggcggcct tcttccaaaa
      841 aattatcatc ttgaaaatga agttgctaga cttaaaaaac ttgttggaga aagaggcggg
      901 agcggcggcc tgctgcccaa gaactaccac ctggagaacg aggtcgcccg cctgaagaag
      961 ctggtcggcg agcgcggtgg ttctggaggt ttgctcccaa agaactacca cttggaaaac
     1021 gaagtcgcaa gattgaagaa gttggtcggt gaaagaggag gaagcggagg attattacct
     1081 aaaaattatc atttggaaaa tgaagttgct agattgaaga agttggttgg agagagaggg
     1141 gggtctggag gactactacc gaaaaactac cacctagaga acgaggtggc ccgactaaaa
     1201 aagctagtgg gcgagcgcgg cggcagtggc ggcctcctcc ccaagaatta ccacctcgaa
     1261 aatgaagtag caaggctcaa gaagctcgta ggagaaagag gtggttccgg gggactcctt
     1321 ccaaaaaact atcatcttga aaacgaagtt gcacgtctta aaaaacttgt tggcgaacgt
     1381 ggcggctctg gcgggctgct gcccaagaat taccacctgg aaaatgaagt ggcccgcctg
     1441 aagaagctgg tgggcgaacg cggaggcagt ggaggcctac taccgaagaa ctaccaccta
     1501 gagaacgagg tggcacgcct aaagaagcta gtgggcgagc gcggcggctc gggtggttta
     1561 ttacctaaga attatcattt agaaaatgaa gtagctcgat taaagaagtt agtaggtgaa
     1621 cgtggtgggt ctggtggtct tcttccgaag aactaccacc ttgagaacga ggtagctcgt
     1681 cttaagaaac ttgtaggtga gcgtggtgga agcggtggtg ggctggcaag ccacgtttgg
     1741 tgccgggagc tgcatgtgtc agaggaactt ttgccaaaga attatcatct tgagaacgaa
     1801 gtggctcgtc ttaagaaact cgtaggagaa agaggtggca gtggaggtct gcttcccaag
     1861 aattaccacc tggaaaatga ggtagctaga ctgaaaaagc tcgtaggcga gcgcggggga
     1921 agtgggggat tgctgccaaa aaattatcat ttggagaacg aagtagcacg actaaagaaa
     1981 ttagttgggg agaggggggg atcgggtggc ttactcccga aaaattatca tctcgaaaac
     2041 gaagtggctc ggctaaaaaa gttagttgga gagcgtggcg gttctggagg cctattacca
     2101 aaaaactacc acctcgaaaa tgaggtggca cgcttaaaaa agctggtcgg agaacgggga
     2161 ggcagtggtg gcctactacc caagaattat catcttgaga acgaggtagc gcgtttgaag
     2221 aagctcgtcg gtgaaagagg tggctcagga gggctgctcc caaagaacta tcatcttgaa
     2281 aatgaggtcg ctcgattaaa aaagttagtg ggcgagaggg gaggcagtgg tgggctactt
     2341 cctaagaatt accacctcga aaacgaagta gctcgattaa agaaattggt cggtgagaga
     2401 ggtgggtcgg gtggcttact gccgaaaaat tatcatctgg aaaatgaggt agcgagacta
     2461 aaaaagctcg taggtgagag agggggttct ggcggcttgc taccaaaaaa ttatcatctt
     2521 gagaacgaag ttgctaggct caaaaagctg gttggagagc gcggcggctc aggcggtctg
     2581 ttacccaaaa actaccactt ggaaaatgag gttgccagat tgaagaaact ggtcggcgag
     2641 cgcggtggaa gtggaggact acttcctaaa aattatcatt tggagaacga agtcgctaga
     2701 ctgaaaaagt tggttgggga acgaggaggg tctggcggca agctagcgat cggatcgatc
     2761 gccgcggaac agcggcgcca tcatacacgc ggccgctaa
//
