LOCUS       sequence-98992-e        2547 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A direct fusion of sfGFP to the 24xGCN4_v4 peptide array.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2547)
  AUTHORS   Tanenbaum ME, Gilbert LA, Qi LS, Weissman JS, Vale RD
  TITLE     A Protein-Tagging System for Signal Amplification in Gene Expression
            and Fluorescence Imaging.
  JOURNAL   Cell. 2014 Oct 8. pii: S0092-8674(14)01227-6. doi: 
            10.1016/j.cell.2014.09.039.
  PUBMED    25307933
REFERENCE   2  (bases 1 to 2547)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2014 
            Oct 8. pii: S0092-8674(14)01227-6. doi: 10.1016/j.cell.2014.09.039."
COMMENT     Sequence Label: pcDNA4TO-24xGCN4_v4-sfGFP
FEATURES             Location/Qualifiers
     source          1..2547
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             70..126
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             142..198
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             214..270
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             286..342
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             358..414
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             430..486
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             502..558
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             574..630
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             646..702
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             718..774
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             790..846
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             862..918
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             934..990
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1006..1062
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1078..1134
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1150..1206
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1222..1278
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1294..1350
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1366..1422
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1438..1494
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1510..1566
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1582..1638
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1726..1782
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1831..2547
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYKG"
     CDS             1831..2547
                     /codon_start=1
                     /product="GFP variant optimized for excitation by UV light"
                     /label=GFPuv
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYKG"
     primer_bind     complement(1858..1886)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     2481..2501
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 atgggcgcca tcatacacgc ggccgcaatc atcggtccgg gtggatctgg aggtggaggt
       61 tctggaggag aagaactttt gagcaagaat tatcatcttg agaacgaagt ggctcgtctt
      121 aagaaaggtt ctggcagtgg agaagaactg ctttcaaaga attaccacct ggaaaatgag
      181 gtagctagac tgaaaaaggg gagcggaagt ggggaggagt tgctgagcaa aaattatcat
      241 ttggagaacg aagtagcacg actaaagaaa gggtccggat cgggtgagga gttactctcg
      301 aaaaattatc atctcgaaaa cgaagtggct cggctaaaaa agggcagtgg ttctggagaa
      361 gagctattat ctaaaaacta ccacctcgaa aatgaggtgg cacgcttaaa aaagggaagt
      421 ggcagtggtg aagagctact atccaagaat tatcatcttg agaacgaggt agcgcgtttg
      481 aagaagggtt ccggctcagg agaggaactg ctctcgaaga actatcatct tgaaaatgag
      541 gtcgctcgat taaaaaaggg atcgggcagt ggtgaggaac tactttcaaa gaattaccac
      601 ctcgaaaacg aagtagctcg attaaagaaa ggttcagggt cgggtgaaga attactgagt
      661 aaaaattatc atctggaaaa tgaggtagcg agactaaaaa aggggagtgg ttctggcgaa
      721 gagttgctat cgaaaaatta tcatcttgag aacgaagttg ctaggctcaa aaagggctca
      781 ggctcaggcg aggagttgct ctcgaaaaac taccacttgg aaaatgaggt cgcgaggttg
      841 aaaaagggga gcgggtcggg cgaggagtta ttgagcaaaa actatcattt agagaacgaa
      901 gtcgcgcgct taaagaaagg ctcgggctcg ggcgaagaac tcttatcgaa gaactaccac
      961 ctcgaaaatg aggtcgccag gttgaaaaag ggcagtggca gcggggagga actcttgagc
     1021 aagaactacc acttggagaa tgaggtcgcg agattgaaga aagggtcggg gagcggcgag
     1081 gaattgctca gcaagaatta tcatttggag aacgaagtcg ccaggctcaa gaaaggctcg
     1141 gggtcggggg aggaattgtt gagtaaaaac taccacttgg aaaatgaagt cgccaggctc
     1201 aaaaaaggga gtgggagcgg cgaagagtta ttgagcaaaa attaccactt ggagaacgaa
     1261 gtggcaaggc tcaagaaagg gagcggcagc ggggaggagc tcttatcgaa gaactaccac
     1321 ttagagaatg aagtcgcccg cttgaagaaa ggctcgggga gcggggaaga gctcttgagc
     1381 aagaactacc acttggaaaa tgaggtggcg cgcttgaaga aagggagcgg gagcggggaa
     1441 gagttactat ctaagaatta tcatctcgag aacgaggtgg ctcgactaaa gaagggctcc
     1501 ggcagtgggg aggaactcct gtcgaagaac tatcatcttg aaaatgaggt tgcaagactt
     1561 aaaaaggggt ccggatcagg tgaggaacta ctcagtaaga attaccacct ggaaaacgaa
     1621 gttgcacgtt tgaagaaagg atcaggatca ggcgaagaac tgctctcaaa agattatcat
     1681 ttggaaaatg aggttgcacg tttaaaaaag ggaagtggca gtggtgagga acttctgtcg
     1741 aaaaattatc atctcgagaa tgaagtagcc cgacttaaaa agggttctgg ctcgggtcag
     1801 cggccgcacc ggtaccccgc ggaaagcggt agcaaaggag aagaactttt cactggagtt
     1861 gtcccaattc ttgttgaatt agatggtgat gttaatgggc acaaattttc tgtccgtgga
     1921 gagggtgaag gtgatgctac aaacggaaaa ctcaccctta aatttatttg cactactgga
     1981 aaactacctg ttccgtggcc aacacttgtc actactctga cctatggtgt tcaatgcttt
     2041 tcccgttatc cggatcacat gaaacggcat gactttttca agagtgccat gcccgaaggt
     2101 tatgtacagg aacgcactat atctttcaaa gatgacggga cctacaagac gcgtgctgaa
     2161 gtcaagtttg aaggtgatac ccttgttaat cgtatcgagt taaagggtat tgattttaaa
     2221 gaagatggaa acattcttgg acacaaactc gagtacaact ttaactcaca caatgtatac
     2281 atcacggcag acaaacaaaa gaatggaatc aaagctaact tcaaaattcg ccacaacgtt
     2341 gaagatggtt ccgttcaact agcagaccat tatcaacaaa atactccaat tggcgatggc
     2401 cctgtccttt taccagacaa ccattacctg tcgacacaat ctgtcctttc gaaagatccc
     2461 aacgaaaagc gtgaccacat ggtccttctt gagtttgtaa ctgctgctgg gattacacat
     2521 ggcatggatg agctctacaa aggttaa
//
