LOCUS       sequence-98995-e        8667 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AML1/ETO9a cDNA was generated by PCR from full-length human 
            AML1/ETO-IRES-GFP and subcloned into MSCV-IRES-GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8667)
  AUTHORS   Zuber J, Radtke I, Pardee TS, Zhao Z, Rappaport AR, Luo W, McCurrach
            ME, Yang MM, Dolan ME, Kogan SC, Downing JR, Lowe SW
  TITLE     Mouse models of human AML accurately predict chemotherapy response.
  JOURNAL   Genes Dev. 2009 Apr 1;23(7):877-89. doi: 10.1101/gad.1771409.
  PUBMED    19339691
REFERENCE   2  (bases 1 to 8667)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1101/gad"; 
            journalName: "Genes Dev"; date: "2009-04-1- 1"; volume: "23"; issue:
            "7"; pages: "877-89"
COMMENT     Sequence Label: MSCV-AML1/ETO-IRES-GFP
FEATURES             Location/Qualifiers
     source          1..8667
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    579..920
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             987..1403
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1333..1355
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1371..1387
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     1413..1429
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1413..1429
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     misc_feature    3761..4222
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3817..3834)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4044..4063
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(4227..4229,4230..4232,4233..4946)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4227..4229,4230..4232,4233..4943)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4272..4293)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4533..4552)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4880..4901
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             5029..5543
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     primer_bind     complement(5712..5728)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5712..5728)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5725..5747)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5736..5752
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5760..5766,5767..5784,5785..5790))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5805..5826
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5943..5960)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6114..6702)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6194..6213)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6873..7664,7665..7733))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7496..7515
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7734..7838)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7906..7924
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7962..7984)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8084..8103
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8297..8319
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8447..8466
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     LTR             join(8666..8667,1..515)
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
ORIGIN
        1 tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat
       61 ggaaaataca taactgagaa tagagaagtt cagatcaagg ttaggaacag agagacagca
      121 gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga
      181 acagatggtc cccagatgcg gtcccgccct cagcagtttc tagagaacca tcagatgttt
      241 ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc
      301 gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc
      361 ctcactcggc gcgccagtcc tccgatagac tgcgtcgccc gggtacccgt attcccaata
      421 aagcctcttg ctgtttgcat ccgaatcgtg gactcgctga tccttgggag ggtctcctca
      481 gattgattga ctgcccacct cgggggtctt tcatttggag gttccaccga gatttggaga
      541 cccctgccca gggaccaccg acccccccgc cgggaggtaa gctggccagc ggtcgtttcg
      601 tgtctgtctc tgtctttgtg cgtgtttgtg ccggcatcta atgtttgcgc ctgcgtctgt
      661 actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac gagttctgaa
      721 cacccggccg caaccctggg agacgtccca gggactttgg gggccgtttt tgtggcccga
      781 cctgaggaag ggagtcgatg tggaatccga ccccgtcagg atatgtggtt ctggtaggag
      841 acgagaacct aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tggaaccgaa
      901 gccgcgcgtc ttgtctgctg cagcgctgca gcatcgttct gtgttgtctc tgtctgactg
      961 tgtttctgta tttgtctgaa aattagggcc agactgttac cactccctta agtttgacct
     1021 taggtcactg gaaagatgtc gagcggatcg ctcacaacca gtcggtagat gtcaagaaga
     1081 gacgttgggt taccttctgc tctgcagaat ggccaacctt taacgtcgga tggccgcgag
     1141 acggcacctt taaccgagac ctcatcaccc aggttaagat caaggtcttt tcacctggcc
     1201 cgcatggaca cccagaccag gtcccctaca tcgtgacctg ggaagccttg gcttttgacc
     1261 cccctccctg ggtcaagccc tttgtacacc ctaagcctcc gcctcctctt cctccatccg
     1321 ccccgtctct cccccttgaa cctcctcgtt cgaccccgcc tcgatcctcc ctttatccag
     1381 ccctcactcc ttctctaggc gccggaattc gttctagaac tagtggatcc gttgtgatgc
     1441 gtatccccgt agatgccagc acgagccgcc gcttcacgcc gccttccacc gcgctgagcc
     1501 caggcaagat gagcgaggcg ttgccgctgg gcgccccgga cgccggcgct gccctggccg
     1561 gcaagctgag gagcggcgac cgcagcatgg tggaggtgct ggccgaccac ccgggcgagc
     1621 tggtgcgcac cgacagcccc aacttcctct gctccgtgct gcctacgcac tggcgctgca
     1681 acaagaccct gcccatcgct ttcaaggtgg tggccctagg ggatgttcca gatggcactc
     1741 tggtcactgt gatggctggc aatgatgaaa actactcggc tgagctgaga aatgctaccg
     1801 cagccatgaa gaaccaggtt gcaagattta atgacctcag gtttgtcggt cgaagtggaa
     1861 gagggaaaag cttcactctg accatcactg tcttcacaaa cccaccgcaa gtcgccacct
     1921 accacagagc catcaaaatc acagtggatg ggccccgaga acctcgaaat cgtactgaga
     1981 agcactccac aatgccagac tcacctgtgg atgtgaagac gcaatctagg ctgactcctc
     2041 caacaatgcc acctccccca actactcaag gagctccaag aaccagttca tttacaccga
     2101 caacgttaac taatggcacg agccattctc ctacagcctt gaatggcgcc ccctcaccac
     2161 ccaatggctt cagcaatggg ccttcctctt cttcctcctc ctctctggct aatcaacagc
     2221 tgcccccagc ctgtggtgcc aggcaactca gcaagctgaa aaggttcctt actaccctgc
     2281 agcagtttgg caatgacatt tcacccgaga taggagaaag agttcgcacc ctcgttctgg
     2341 gactagtgaa ctccactttg acaattgaag aatttcattc caaactgcaa gaagctacta
     2401 acttcccact gagacctttt gtcatcccat ttttgaaggc caacttgccc ctgctgcagc
     2461 gtgagctcct ccactgcgca agactggcca aacagaaccc tgcccagtac ctcgcccagc
     2521 atgaacagct gcttctggat gccagcacca cctcacctgt tgactcctca gagctgcttc
     2581 tcgatgtgaa cgaaaacggg aagaggcgaa ctccagacag aaccaaagaa aatggctttg
     2641 acagagagcc tttgcactca gaacatccaa gcaagcgacc atgcactatt agcccaggcc
     2701 agcggtacag tccaaataac ggcttatcct accagcccaa tggcctgcct caccctaccc
     2761 cacctccacc tcagcattac cgtttggatg atatggccat tgcccaccac tacagggact
     2821 cctatcgaca ccccagccac agggacctca gggacagaaa cagacctatg gggttgcatg
     2881 gcacacgtca agaagaaatg attgatcaca gactaacaga cagagaatgg gcagaagagt
     2941 ggaaacatct tgaccatctg ttaaactgca taatggacat ggtagaaaaa acaaggcgat
     3001 ctctcaccgt actaaggcgg tgtcaagaag cagaccggga agaattgaat tactggatcc
     3061 ggcggtacag tgacgccgag gacttaaaaa aaggtggcgg cagtagcagc agccactcta
     3121 ggcagcagag tcccgtcaac ccagacccag ttgcactaga cgcgcatcgg gaattccttc
     3181 acaggcctgc gtctggatac gtgccagagg agatctggaa gaaagctgag gaggccgtca
     3241 atgaggtgaa gcgccaggcg atgacggagc tgcagaaggc cgtgtctgag gcggagcgga
     3301 aagcccacga catgatcaca acagagaggg ccaagatgga gcgcacggtc gccgaggcca
     3361 aacggcaggc ggcggaggac gcactggcag ttatcaatca gcaggaggat tcaagcgaga
     3421 gttgctggaa ttgtggccgt aaagcgagtg aaacctgcag tggctgtaac acagcccgat
     3481 actgtggctc attttgccag cacaaagact gggagaagca ccatcacatc tgtggacaga
     3541 ccctgcaggc ccagcagcag ggagacacac ctgcagtcag ctcctctgtc acgcccaaca
     3601 gcggggctgg gagcccgatg gacacaccac cagcagccac tccgaggtca accaccccgg
     3661 gaaccccttc caccatagag acaacccctc gctctcgact ctagaacctc gagaaaactc
     3721 gacggttatt ttccaccata ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc
     3781 ctgtcttctt gacgagcatt cctaggggtc tttcccctct cgccaaagga atgcaaggtc
     3841 tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa acaacgtctg
     3901 tagcgaccct ttgcaggcag cggaaccccc cacctggcga caggtgcctc tgcggccaaa
     3961 agccacgtgt ataagataca cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt
     4021 ggatagttgt ggaaagagtc aaatggctct cctcaagcgt attcaacaag gggctgaagg
     4081 atgcccagaa ggtaccccat tgtatgggat ctgatctggg gcctcggtgc acatgcttta
     4141 catgtgttta gtcgaggtta aaaaacgtct aggccccccg aaccacgggg acgtggtttt
     4201 cctttgaaaa acacgatgat aataccatgg tgagcaaggg cgaggagctg ttcaccgggg
     4261 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
     4321 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg
     4381 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct
     4441 tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
     4501 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
     4561 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
     4621 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct
     4681 atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca
     4741 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
     4801 gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc
     4861 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
     4921 tcggcatgga cgagctgtac aagtaaagcg gccaactcga cgttattccc cttcgaaggc
     4981 ctatcgataa aataaaagat tttatttagt ctccagaaaa aggggggaat gaaagacccc
     5041 acctgtaggt ttggcaagct agcttaagta acgccatttt gcaaggcatg gaaaatacat
     5101 aactgagaat agagaagttc agatcaaggt taggaacaga gagacagcag aatatgggcc
     5161 aaacaggata tctgtggtaa gcagttcctg ccccggctca gggccaagaa cagatggtcc
     5221 ccagatgcgg tcccgccctc agcagtttct agagaaccat cagatgtttc cagggtgccc
     5281 caaggacctg aaatgaccct gtgccttatt tgaactaacc aatcagttcg cttctcgctt
     5341 ctgttcgcgc gcttctgctc cccgagctca ataaaagagc ccacaacccc tcactcggcg
     5401 cgccagtcct ccgatagact gcgtcgcccg ggtacccgtg tatccaataa accctcttgc
     5461 agttgcatcc gacttgtggt ctcgctgttc cttgggaggg tctcctctga gtgattgact
     5521 acccgtcagc gggggtcttt catgggtaac agtttcttga agttggagaa caacattctg
     5581 agggtaggag tcgaatatta agtaatcctg actcaattag ccactgtttt gaatccacat
     5641 actccaatac tcctgaaata gttcattatg gacagcgcag aagagctggg gagaattaat
     5701 tcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     5761 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     5821 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     5881 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     5941 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     6001 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     6061 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     6121 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     6181 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     6241 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     6301 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     6361 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     6421 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     6481 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     6541 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     6601 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     6661 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     6721 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     6781 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     6841 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     6901 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     6961 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     7021 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     7081 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     7141 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     7201 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     7261 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     7321 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     7381 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     7441 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     7501 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     7561 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     7621 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     7681 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     7741 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     7801 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     7861 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
     7921 aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc
     7981 ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc
     8041 gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt
     8101 gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac
     8161 cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg
     8221 gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg
     8281 gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggcgcaagg aatggtgcat
     8341 gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga
     8401 aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga
     8461 tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt
     8521 agaggcgatt agtccaattt gttaaagaca ggatatcagt ggtccaggct ctagttttga
     8581 ctcaacaata tcaccagctg aagcctatag agtacgagcc atagataaaa taaaagattt
     8641 tatttagtct ccagaaaaag gggggaa
//
