LOCUS       sequence-98998-e        6723 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MethyloBrick expression vector, fumC promoter; TcR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6723)
  AUTHORS   Schada von Borzyskowski L, Remus-Emsermann M, Weishaupt R, Vorholt 
            JA, Erb TJ
  TITLE     A Set of Versatile Brick Vectors and Promoters for the Assembly, 
            Expression, and Integration of Synthetic Operons in Methylobacterium
            extorquens AM1 and Other Alphaproteobacteria.
  JOURNAL   ACS Synth Biol. 2014 Aug 8.
  PUBMED    25105793
REFERENCE   2  (bases 1 to 6723)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2014 Aug 8."
COMMENT     Sequence Label: pTE103
FEATURES             Location/Qualifiers
     source          1..6723
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      11..642
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      1105..1693
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1594..1613
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(2010..2027)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2010..2026)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2019..2041)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(2235..2254)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2354..2376
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2414..2432)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             3218..4417
                     /codon_start=1
                     /gene="tetA"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     CDS             complement(4648..5796)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(6068..6439)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSL*AYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            6472..6581
                     /note="incP origin of transfer"
ORIGIN
        1 gaccctttcc gacgctcacc gggctggttg ccctcgccgc tgggctggcg gccgtctatg
       61 gccctgcaaa cgcgccagaa acgccgtcga agccgtgtgc gagacaccgc ggccgccggc
      121 gttgtggata cctcgcggaa aacttggccc tcactgacag atgaggggcg gacgttgaca
      181 cttgaggggc cgactcaccc ggcgcggcgt tgacagatga ggggcaggct cgatttcggc
      241 cggcgacgtg gagctggcca gcctcgcaaa tcggcgaaaa cgcctgattt tacgcgagtt
      301 tcccacagat gatgtggaca agcctgggga taagtgccct gcggtattga cacttgaggg
      361 gcgcgactac tgacagatga ggggcgcgat ccttgacact tgaggggcag agtgctgaca
      421 gatgaggggc gcacctattg acatttgagg ggctgtccac aggcagaaaa tccagcattt
      481 gcaagggttt ccgcccgttt ttcggccacc gctaacctgt cttttaacct gcttttaaac
      541 caatatttat aaaccttgtt tttaaccagg gctgcgccct gtgcgcgtga ccgcgcacgc
      601 cgaagggggg tgccccccct tctcgaaccc tcccggcccg ctaacgcggg cctcccatcc
      661 ccccaggggc tgcgcccctc ggccgcgaac ggcctcaccc caaaaatggc agccaagctg
      721 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
      781 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
      841 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
      901 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
      961 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     1021 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     1081 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     1141 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     1201 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt
     1261 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     1321 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     1381 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     1441 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     1501 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     1561 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     1621 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     1681 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     1741 ttttgctcac atggaattct gtacatctag acgtgagcgg cggcggcacc ttgggccgga
     1801 aaagcggtgt ggtcgtcgga ggcgcgaggg gcagcgtatc cagcatcggc aggcgaacaa
     1861 cctcggcggt aacggagtgc gcgatgcgtc atcccccgtt cggggccggc atcgcgcttg
     1921 tcatcgtgct tcgttaagta gggccgcatc agagccaacg aagaggccac gaagcttaga
     1981 tcttgactag tcctgcaggt acctaattca ctggccgtcg ttttacaacg tcgtgactgg
     2041 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     2101 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     2161 gaatggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata
     2221 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg
     2281 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa
     2341 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc
     2401 gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
     2461 gtttcttagc accctttctc ggtccttcaa cgttcctgac aacgagcctc cttttcgcca
     2521 atccatcgac aatcaccgcg agtccctgct cgaacgctgc gtccggaccg gcttcgtcga
     2581 aggcgtctat cgcggcccgc aacagcggcg agagcggagc ctgttcaacg gtgccgccgc
     2641 gctcgccggc atcgctgtcg ccggcctgct cctcaagcac ggccccaaca gtgaagtagc
     2701 tgattgtcat cagcgcattg acggcgtccc cggccgaaaa acccgcctcg cagaggaagc
     2761 gaagctgcgc gtcggccgtt tccatctgcg gtgcgcccgg tcgcgtgccg gcatggatgc
     2821 gcgcgccatc gcggtaggcg agcagcgcct gcctgaagct gcgggcattc ccgatcagaa
     2881 atgagcgcca gtcgtcgtcg gctctcggca ccgaatgcgt atgattctcc gccagcatgg
     2941 cttcggccag tgcgtcgagc agcgcccgct tgttcctgaa gtgccagtaa agcgccggct
     3001 gctgaacccc caaccgttcc gccagtttgc gtgtcgtcag accgtctacg ccgacctcgt
     3061 tcaacaggtc cagggcggca cggatcactg tattcggctg caactttgtc atgcttgaca
     3121 ctttatcact gataaacata atatgtccac caacttatca gtgataaaga atccgcgcgt
     3181 tcaatcggac cagcggaggc tggtccggag gccagacgtg aaacccaaca tacccctgat
     3241 cgtaattctg agcactgtcg cgctcgacgc tgtcggcatc ggcctgatta tgccggtgct
     3301 gccgggcctc ctgcgcgatc tggttcactc gaacgacgtc accgcccact atggcattct
     3361 gctggcgctg tatgcgttgg tgcaatttgc ctgcgcacct gtgctgggcg cgctgtcgga
     3421 tcgtttcggg cggcggccaa tcttgctcgt ctcgctggcc ggcgccactg tcgactacgc
     3481 catcatggcg acagcgcctt tcctttgggt tctctatatc gggcggatcg tggccggcat
     3541 caccggggcg actggggcgg tagccggcgc ttatattgcc gatatcactg atggcgatga
     3601 gcgcgcgcgg cacttcggct tcatgagcgc ctgtttcggg ttcgggatgg tcgcgggacc
     3661 tgtgctcggt gggctgatgg gcggtttctc cccccacgct ccgttcttcg ccgcggcagc
     3721 cttgaacggc ctcaatttcc tgacgggctg tttccttttg ccggagtcgc acaaaggcga
     3781 acgccggccg ttacgccggg aggctctcaa cccgctcgct tcgttccggt gggcccgggg
     3841 catgaccgtc gtcgccgccc tgatggcggt cttcttcatc atgcaacttg tcggacaggt
     3901 gccggccgcg ctttgggtca ttttcggcga ggatcgcttt cactgggacg cgaccacgat
     3961 cggcatttcg cttgccgcat ttggcattct gcattcactc gcccaggcaa tgatcaccgg
     4021 ccctgtagcc gcccggctcg gcgaaaggcg ggcactcatg ctcggaatga ttgccgacgg
     4081 cacaggctac atcctgcttg ccttcgcgac acggggatgg atggcgttcc cgatcatggt
     4141 cctgcttgct tcgggtggca tcggaatgcc ggcgctgcaa gcaatgttgt ccaggcaggt
     4201 ggatgaggaa cgtcaggggc agctgcaagg ctcactggcg gcgctcacca gcctgacctc
     4261 gatcgtcgga cccctcctct tcacggcgat ctatgcggct tctataacaa cgtggaacgg
     4321 gtgggcatgg attgcaggcg ctgccctcta cttgctctgc ctgccggcgc tgcgtcgcgg
     4381 gctttggagc ggcgcagggc aacgagccga tcgctgatcg tggaaacgat aggcctatgc
     4441 catgcgggtc aaggcgactt ccggcaagct atacgcgccc tagaattgtc aattttaatc
     4501 ctctgtttat cggcagttcg tagagcgcgc cgtgcgtccc gagcgatact gagcgaagca
     4561 agtgcgtcga gcagtgcccg cttgttcctg aaatgccagt aaagcgctgg ctgctgaacc
     4621 cccagccgga actgacccca caaggcccta gcgtttgcaa tgcaccaggt catcattgac
     4681 ccaggcgtgt tccaccaggc cgctgcctcg caactcttcg caggcttcgc cgacctgctc
     4741 gcgccacttc ttcacgcggg tggaatccga tccgcacatg aggcggaagg tttccagctt
     4801 gagcgggtac ggctcccggt gcgagctgaa atagtcgaac atccgtcggg ccgtcggcga
     4861 cagcttgcgg tacttctccc atatgaattt cgtgtagtgg tcgccagcaa acagcacgac
     4921 gatttcctcg tcgatcagga cctggcaacg ggacgttttc ttgccacggt ccaggacgcg
     4981 gaagcggtgc agcagcgaca ccgattccag gtgcccaacg cggtcggacg tgaagcccat
     5041 cgccgtcgcc tgtaggcgcg acaggcattc ctcggccttc gtgtaatacc ggccattgat
     5101 cgaccagccc aggtcctggc aaagctcgta gaacgtgaag gtgatcggct cgccgatagg
     5161 ggtgcgcttc gcgtactcca acacctgctg ccacaccagt tcgtcatcgt cggcccgcag
     5221 ctcgacgccg gtgtaggtga tcttcacgtc cttgttgacg tggaaaatga ccttgttttg
     5281 cagcgcctcg cgcgggattt tcttgttgcg cgtggtgaac agggcagagc gggccgtgtc
     5341 gtttggcatc gctcgcatcg tgtccggcca cggcgcaata tcgaacaagg aaagctgcat
     5401 ttccttgatc tgctgcttcg tgtgtttcag caacgcggcc tgcttggcct cgctgacctg
     5461 ttttgccagg tcctcgccgg cggtttttcg cttcttggtc gtcatagttc ctcgcgtgtc
     5521 gatggtcatc gacttcgcca aacctgccgc ctcctgttcg agacgacgcg aacgctccac
     5581 ggcggccgat ggcgcgggca gggcaggggg agccagttgc acgctgtcgc gctcgatctt
     5641 ggccgtagct tgctggacca tcgagccgac ggactggaag gtttcgcggg gcgcacgcat
     5701 gacggtgcgg cttgcgatgg tttcggcatc ctcggcggaa aaccccgcgt cgatcagttc
     5761 ttgcctgtat gccttccggt caaacgtccg attcattcac cctccttgcg ggattgcccc
     5821 gactcacgcc ggggcaatgt gcccttattc ctgatttgac ccgcctggtg ccttggtgtc
     5881 cagataatcc accttatcgg caatgaagtc ggtcccgtag accgtctggc cgtccttctc
     5941 gtacttggta ttccgaatct tgccctgcac gaataccagc tccgcgaagt cgctcttctt
     6001 gatggagcgc atggggacgt gcttggcaat cacgcgcacc ccccggccgt tttagcggct
     6061 aaaaaagtca tggctctgcc ctcgggcgga ccacgcccat catgaccttg ccaagctcgt
     6121 cctgcttctc ttcgatcttc gccagcaggg cgaggatcgt ggcatcaccg aaccgcgccg
     6181 tgcgcgggtc gtcggtgagc cagagtttca gcaggccgcc caggcggccc aggtcgccat
     6241 tgatgcgggc cagctcgcgg acgtgctcat agtccacgac gcccgtgatt ttgtagccct
     6301 ggccgacggc cagcaggtag gcctacaggc tcatgccggc cgccgccgcc ttttcctcaa
     6361 tcgctcttcg ttcgtctgga aggcagtaca ccttgatagg tgggctgccc ttcctggttg
     6421 gcttggtttc atcagccatc cgcttgccct catctgttac gccggcggta gccggccagc
     6481 ctcgcagagc aggattcccg ttgagcaccg ccaggtgcga ataagggaca gtgaagaagg
     6541 aacacccgct cgcgggtggg cctacttcac ctatcctgcc cggctgacgc cgttggatac
     6601 accaaggaaa gtctacacga accctttggc aaaatcctgt atatcgtgcg aaaaaggatg
     6661 gatataccga aaaaatcgct ataatgaccc cgaagcaggg ttatgcagcg gaaaagatcc
     6721 gtc
//
