LOCUS       sequence-99028-e         481 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of two gRNAs from Drosophila U6:1 and U6:3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 481)
  AUTHORS   Port F, Chen HM, Lee T, Bullock SL
  TITLE     Optimized CRISPR/Cas tools for efficient germline and somatic genome
            engineering in Drosophila.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Jul 7. pii: 201405500.
  PUBMED    25002478
REFERENCE   2  (bases 1 to 481)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2014 Jul 7. pii: 201405500."
COMMENT     Sequence Label: pCFD4-U6:1_U6:3tandemgRNAs
FEATURES             Location/Qualifiers
     source          1..481
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 cctgtggcgg ctacgaaaat ctccccggcc atgtcggcct ccacctccag cgaaaaaccc
       61 atcagcgagc tggccacctc tgtgctgacc caccgctttc cagactccac ctcctcaccc
      121 ggcgaacatg gccttggacg aatgcagttg tcgatccgct acagcgccca gcgtcaaaaa
      181 ctagacgtga ccatacacaa aatccagaag ataccacttc gcgatcccag caatatcccc
      241 gatccgtatg ttaagctgta tctgttgcct ggacgcacca aggagtcgaa acgcaagacg
      301 agcgtgatca aggacaactg caaccccgtc tacgatgcat cctttgagta cctgatttcc
      361 attgccgaac tcaggcagac ggaactggag gtgacggtgt gcacccaaaa gggattccta
      421 tccggcggta gtcccatcat tggcatggta ggtacccgaa agcaacccct tagttacaga
      481 c
//
