LOCUS       sequence-99029-e         728 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of two gRNAs from Drosophila U6:1 and U6:3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 728)
  AUTHORS   Port F, Chen HM, Lee T, Bullock SL
  TITLE     Optimized CRISPR/Cas tools for efficient germline and somatic genome
            engineering in Drosophila.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Jul 7. pii: 201405500.
  PUBMED    25002478
REFERENCE   2  (bases 1 to 728)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2014 Jul 7. pii: 201405500."
COMMENT     Sequence Label: pCFD4-U6:1_U6:3tandemgRNAs
FEATURES             Location/Qualifiers
     source          1..728
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    522..591
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
ORIGIN
        1 tcggacagca tacactgggc atccagcagt ttgtccagca tcagatactc tccatagatt
       61 ttgcccactt ccgtggttaa tggcaccgcc gaatcatcgt gatcgtttct gtatgggttt
      121 gaattgaatc gcagaactga agatcgattg gcattcctgg acagcacgtg ctggtgctca
      181 cccgtttcct gcatagggac agctcatggt gcacagctca gatcagatcg tgactcctcg
      241 agcggcggat gctggcgaac tgatctccgc cagcggaccg gagatgagac cccagcgaac
      301 cgataacaga gcgagagagc tccagttccg actgattgca cagtcggtga tctgggcgat
      361 gggcactgcc agataggctg ggaattatca atcacttgag gtgaaagtgc ggcgcacaca
      421 aatccaagct tgatatcatc gatctcgagg ctgcatccaa cgcgttggga gctctccgga
      481 tcaattcggc ttcaggtacc gtcgacgatg taggtcacgg tctcgaagcc gcggtgcggg
      541 tgccagggcg tgcccttggg ctccccgggc gcgtactcca cctcacccat ctggtccatc
      601 atgatgaacg ggtcgaggtg gcggtagttg atcccggcga acgcgcggcg caccgggaag
      661 ccctcgccct cgaaaccgct gggcgcggtg gtcacggtga gcacgggacg tgcgacggcg
      721 tcggctgg
//
