LOCUS       sequence-99032-e         405 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of two gRNAs from Drosophila U6:1 and U6:3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 405)
  AUTHORS   Port F, Chen HM, Lee T, Bullock SL
  TITLE     Optimized CRISPR/Cas tools for efficient germline and somatic genome
            engineering in Drosophila.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Jul 7. pii: 201405500.
  PUBMED    25002478
REFERENCE   2  (bases 1 to 405)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2014 Jul 7. pii: 201405500."
COMMENT     Sequence Label: pCFD4-U6:1_U6:3tandemgRNAs
FEATURES             Location/Qualifiers
     source          1..405
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 caacgcatag ccaaacattg acgaattgga taccctgccg attgtcagac acttttgttg
       61 atcagtttct tgcgaatggt ctcgtccagc ggtggaatcg cctcgcgggg aatcagaaaa
      121 gtggacagat tgaacagatc cagaaacacc ttgtaccgat cactgaaacc aaaaaaaaac
      181 aaagggagaa cagtttgagt tcattgatcc ccgatataat cacatctgcg atgatcacct
      241 gagagtggag cgcagatatt gatatccaga cgagccacca gtgcccaact gttgggatcc
      301 aatcatgcgt tgcaccatga tcacgtgatt gtctgcggcg ggaatagaaa gtatttggtt
      361 aggaaaacca gtcttaaaca taagatatat ttataaaaga gtatc
//
