LOCUS       sequence-99038-e        6225 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Luc reporter driven by 1802 bp of the CIP2A promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6225)
  AUTHORS   Khanna A, Okkeri J, Bilgen T, Tiirikka T, Vihinen M, Visakorpi T, 
            Westermarck J
  TITLE     ETS1 mediates MEK1/2-dependent overexpression of cancerous inhibitor
            of protein phosphatase 2A (CIP2A) in human cancer cells.
  JOURNAL   PLoS One. 2011 Mar 22;6(3):e17979. doi: 
            10.1371/journal.pone.0017979.
  PUBMED    21445343
REFERENCE   2  (bases 1 to 6225)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011-03-22"; volume: "6(3):e17979. doi"; pages: " 
            10.1371/journal.pone.0017979"
COMMENT     Sequence Label: CIP2Aprom1802bp-pGL4.10Luc
FEATURES             Location/Qualifiers
     source          1..6225
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      2077..2086
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2083..3735
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    3779..3900
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3790..3809)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3844..3863
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(4148..4165)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4319..4907)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4399..4418)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5107..5898,5899..5967))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    6072..6120
                     /note="synthetic polyadenylation signal"
     misc_feature    6134..6225
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     6174..6193
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 ggcctaactg gccggtacct gagctcgcta gcctcgagtc ctctcctcct cgttctttct
       61 acactagcca catggcatcc ttgtttctgg catacagtat cccactttag ggtctttctt
      121 tgccatttcc tctatctaaa aaccttttcc catagaatat ggctcccttt ctcaactcct
      181 tgaagcattt ccccaaatgt tgctttctta atgaggtcac cttcccattt cccttatcaa
      241 ttaccaacat tattctagag taataacgtc tatttttctc tttatatcat tttccttact
      301 tatttattgt gtttattatt tggttcccta ccaaaatgta agttgcatgc cagtagtatt
      361 tttgtttttt tcactgagtc tattgtgtct agaaaagtgc ttagcacatt gtagattctc
      421 aatgaactac ttattgaatg aacagtccta tgaaccaggt atctcccttg gccagatttt
      481 acctaatgaa gattctgcag cagtgagaac ttgcctagag tcacatcgtc aaaggtggag
      541 ctagaatctg taagcaacct ggctctctac tctttaccca ctgctgcatg ttactgcatg
      601 gtgcctctca tttatgtggt gaatttcaaa agtactattt tgtatgtttc ccttactaga
      661 cagttccctc gcagcagggg atactacttc atctctgttc cctgactggc agtttctaac
      721 ccatgttaca cgctctataa ttgtttacgg aatgaatgaa agcataaatg aacacacaag
      781 ctacccaaag atttttacat gaatgtccca actggttaaa tcatttttat ctttcagatt
      841 taaactttta taaaaaacaa aaccaaaaca ccaaacctgt cttcaaaaat ctagtaattc
      901 aaaaaaattc ttttgaagtg aaaaaacatg atttctgata aacgatttaa ttaaatggaa
      961 atgtacccta ttcttacaat ttctacatcc tggtttttaa agctttattc gacgttagga
     1021 aatattaagc aaaaagctcc aaaaccagta actgactcgc ttcattacat acacatgcaa
     1081 taatattaag ctatctccga tttaaaaaac gcaaataata gtgccatttt cgtagacatc
     1141 gagaattata aaacaatcaa tcatgccaga gagaaacaga caccttcact tacaaaaact
     1201 gacgctaacg agtaacaccc tccggagcaa tacctggccc agggtcagca ggaggtagtc
     1261 ttctcgaata aatgtgacaa atactttggt cagggcatga ctaatttgcc acataatgcc
     1321 aataggttga ctctgagaaa ctaccatccc cgggcagaag gggcgggtgt aggagagatt
     1381 taaaaacgag gcccctccca ggccgcctca aaatctaggg cccaagattc ttctcctcag
     1441 agctcctcac cgtgtctaga ctaggggcaa gcgaccattt ctcaggtaga ggatgacgca
     1501 caaacgaaaa actggccatc caacatgaag gacgaggaag cgcacgaaat cagagcgcac
     1561 agctgagtga ggaatccccc ttctcctaac cgattcctct cccgagaaat cgcgagattt
     1621 ctcgccttca cgggatctca ggccgaaacc tcgcggcctc tcagacgagg gtgggttagc
     1681 gggggcagct cccaaccccc gtcctggacc cacaaatcac ctcgacccct ggcccacccc
     1741 ggccgtcacc gagaacggtc ccctagggtg cctagggact tccggagccc gaccggatcc
     1801 ggaagcttct gagagcgagg gggtggggcc gaaaatcaaa aaaagcgcgg cgaaagctaa
     1861 aggccggcgc acgctgggcg gtggtggtcc ctaagccggg ccgcggccgg tgcaatggac
     1921 tccactgcct gcttgaagtc cttgctcctg actgtcagtc agtacaaagc cgtgaagtca
     1981 gaggcgaacg ccactcagct tttgcggcac ttggaggtaa tttctggaag atctggcctc
     2041 ggcggccaag cttggcaatc cggtactgtt ggtaaagcca ccatggaaga tgccaaaaac
     2101 attaagaagg gcccagcgcc attctaccca ctcgaagacg ggaccgccgg cgagcagctg
     2161 cacaaagcca tgaagcgcta cgccctggtg cccggcacca tcgcctttac cgacgcacat
     2221 atcgaggtgg acattaccta cgccgagtac ttcgagatga gcgttcggct ggcagaagct
     2281 atgaagcgct atgggctgaa tacaaaccat cggatcgtgg tgtgcagcga gaatagcttg
     2341 cagttcttca tgcccgtgtt gggtgccctg ttcatcggtg tggctgtggc cccagctaac
     2401 gacatctaca acgagcgcga gctgctgaac agcatgggca tcagccagcc caccgtcgta
     2461 ttcgtgagca agaaagggct gcaaaagatc ctcaacgtgc aaaagaagct accgatcata
     2521 caaaagatca tcatcatgga tagcaagacc gactaccagg gcttccaaag catgtacacc
     2581 ttcgtgactt cccatttgcc acccggcttc aacgagtacg acttcgtgcc cgagagcttc
     2641 gaccgggaca aaaccatcgc cctgatcatg aacagtagtg gcagtaccgg attgcccaag
     2701 ggcgtagccc taccgcaccg caccgcttgt gtccgattca gtcatgcccg cgaccccatc
     2761 ttcggcaacc agatcatccc cgacaccgct atcctcagcg tggtgccatt tcaccacggc
     2821 ttcggcatgt tcaccacgct gggctacttg atctgcggct ttcgggtcgt gctcatgtac
     2881 cgcttcgagg aggagctatt cttgcgcagc ttgcaagact ataagattca atctgccctg
     2941 ctggtgccca cactatttag cttcttcgct aagagcactc tcatcgacaa gtacgaccta
     3001 agcaacttgc acgagatcgc cagcggcggg gcgccgctca gcaaggaggt aggtgaggcc
     3061 gtggccaaac gcttccacct accaggcatc cgccagggct acggcctgac agaaacaacc
     3121 agcgccattc tgatcacccc cgaaggggac gacaagcctg gcgcagtagg caaggtggtg
     3181 cccttcttcg aggctaaggt ggtggacttg gacaccggta agacactggg tgtgaaccag
     3241 cgcggcgagc tgtgcgtccg tggccccatg atcatgagcg gctacgttaa caaccccgag
     3301 gctacaaacg ctctcatcga caaggacggc tggctgcaca gcggcgacat cgcctactgg
     3361 gacgaggacg agcacttctt catcgtggac cggctgaaga gcctgatcaa atacaagggc
     3421 taccaggtag ccccagccga actggagagc atcctgctgc aacaccccaa catcttcgac
     3481 gccggggtcg ccggcctgcc cgacgacgat gccggcgagc tgcccgccgc agtcgtcgtg
     3541 ctggaacacg gtaaaaccat gaccgagaag gagatcgtgg actatgtggc cagccaggtt
     3601 acaaccgcca agaagctgcg cggtggtgtt gtgttcgtgg acgaggtgcc taaaggactg
     3661 accggcaagt tggacgcccg caagatccgc gagattctca ttaaggccaa gaagggcggc
     3721 aagatcgccg tgtaataatt ctagagtcgg ggcggccggc cgcttcgagc agacatgata
     3781 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt
     3841 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt
     3901 aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt
     3961 taaagcaagt aaaacctcta caaatgtggt aaaatcgata aggatccgtc gaccgatgcc
     4021 cttgagagcc ttcaacccag tcagctcctt ccggtgggcg cggggcatga ctatcgtcgc
     4081 cgcacttatg actgtcttct ttatcatgca actcgtagga caggtgccgg cagcgctctt
     4141 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     4201 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     4261 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     4321 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     4381 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     4441 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     4501 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     4561 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     4621 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     4681 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     4741 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     4801 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     4861 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     4921 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     4981 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     5041 caatctaaag tatatatgag taaacttggt ctgacagcgg ccgcaaatgc taaaccactg
     5101 cagtggttac cagtgcttga tcagtgaggc accgatctca gcgatctgcc tatttcgttc
     5161 gtccatagtg gcctgactcc ccgtcgtgta gatcactacg attcgtgagg gcttaccatc
     5221 aggccccagc gcagcaatga tgccgcgaga gccgcgttca ccggcccccg atttgtcagc
     5281 aatgaaccag ccagcaggga gggccgagcg aagaagtggt cctgctactt tgtccgcctc
     5341 catccagtct atgagctgct gtcgtgatgc tagagtaaga agttcgccag tgagtagttt
     5401 ccgaagagtt gtggccattg ctactggcat cgtggtatca cgctcgtcgt tcggtatggc
     5461 ttcgttcaac tctggttccc agcggtcaag ccgggtcaca tgatcaccca tattatgaag
     5521 aaatgcagtc agctccttag ggcctccgat cgttgtcaga agtaagttgg ccgcggtgtt
     5581 gtcgctcatg gtaatggcag cactacacaa ttctcttacc gtcatgccat ccgtaagatg
     5641 cttttccgtg accggcgagt actcaaccaa gtcgttttgt gagtagtgta tacggcgacc
     5701 aagctgctct tgcccggcgt ctatacggga caacaccgcg ccacatagca gtactttgaa
     5761 agtgctcatc atcgggaatc gttcttcggg gcggaaagac tcaaggatct tgccgctatt
     5821 gagatccagt tcgatatagc ccactcttgc acccagttga tcttcagcat cttttacttt
     5881 caccagcgtt tcggggtgtg caaaaacagg caagcaaaat gccgcaaaga agggaatgag
     5941 tgcgacacga aaatgttgga tgctcatact cgtccttttt caatattatt gaagcattta
     6001 tcagggttac tagtacgtct ctcaaggata agtaagtaat attaaggtac gggaggtatt
     6061 ggacaggccg caataaaata tctttatttt cattacatct gtgtgttggt tttttgtgtg
     6121 aatcgatagt actaacatac gctctccatc aaaacaaaac gaaacaaaac aaactagcaa
     6181 aataggctgt ccccagtgca agtgcaggtg ccagaacatt tctct
//
