LOCUS       sequence-9906-ex        4017 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4017)
  AUTHORS   Zeiser E, Frokjaer-Jensen C, Jorgensen E, Ahringer J
  TITLE     MosSCI and gateway compatible plasmid toolkit for constitutive and 
            inducible expression of transgenes in the C. elegans germline.
  JOURNAL   PLoS One. 2011;6(5):e20082. Epub 2011 May 26.
  PUBMED    21637852
REFERENCE   2  (bases 1 to 4017)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(5)"; pages: "e20082. Epub 2011 May 26"
COMMENT     Sequence Label: pJA254
FEATURES             Location/Qualifiers
     source          1..4017
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    593..688
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1207..1235)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     1983..2003
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     protein_bind    2063..2186
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2258..2277)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2259..2277)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2282..2298)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2282..2298)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2400..2419)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2411..3220
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2478..2497)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3367..3955
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3856..3875
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccgc gttaacgcta ccatggagct ccaaataatg
      601 attttatttt gactgatagt gacctgttcg ttgcaacaaa ttgataagca atgctttttt
      661 ataatgccaa ctttgtatag aaaagttgaa atatcagttt ttaaaaaatt aaaccataaa
      721 acaaataata taacccaatt tttacatcaa accacaagaa aaaaatacat ttgggcccac
      781 ggataaagaa attaaaaaaa tacatttttt aaaggcgcac cgaattaaaa ttcatttggg
      841 tcttaccgcg tataccgtac tccgtttgtt tgatcatttt tgtcagcgct ggcggttgtt
      901 ttttcatttc atttctgctt caaagacgtt ttctcgaata atttttcgtt tattctcttt
      961 tttaaaatta atttctagcc gtaaatgtta taaattcacc catttaacgc aaatttcatg
     1021 gtaatctcat ggaaaaatgc agtttctttg ttaaagaaag cttaaatagc aaaaattccc
     1081 cgactttccc caaaatcctg ctcgattttc cgttttctca ttgtattctc tcttaattaa
     1141 ttttatcgat aatcaattga atgtttcaga cagagaatga gtaaaggaga agaacttttc
     1201 actggagttg tcccaattct tgttgaatta gatggtgatg ttaatgggca caaattttct
     1261 gtcagtggag agggtgaagg tgatgcaaca tacggaaaac ttacccttaa atttatttgc
     1321 actactggaa aactacctgt tccatgggta agtttaaaca tatatatact aactaaccct
     1381 gattatttaa attttcagcc aacacttgtc actactctca cttatggtgt tcaatgcttc
     1441 tcgagatacc cagatcatat gaaacagcat gactttttca agagtgccat gcccgaaggt
     1501 tatgtacagg aaagaactat atttttcaaa gatgacggga actacaagac acgtaagttt
     1561 aaacagttcg gtactaacta accatacata tttaaatttt caggtgctga agtcaagttt
     1621 gaaggtgata cccttgttaa tagaatcgag ttaaaaggta ttgattttaa agaagatgga
     1681 aacattcttg gacacaaatt ggaatacaac tataactcac acaatgtata catcatggca
     1741 gacaaacaaa agaatggaat caaagttgta agtttaaaca tgattttact aactaactaa
     1801 tctgatttaa attttcagaa cttcaaaatt agacacaaca ttgaagatgg aagcgttcaa
     1861 ctagcagacc attatcaaca aaatactcca attggcgatg gccctgtcct tttaccagac
     1921 aaccattacc tgtccacaca atctgccctt tcgaaagatc ccaacgaaaa gagagaccac
     1981 atggtccttc ttgagtttgt aacagctgct gggattacac atggcatgga cgaactatac
     2041 aaaggaggtg gaggtggagc ttcaagtttg tacaaaaaag ttgaacgaga aacgtaaaat
     2101 gatataaata tcaatatatt aaattagatt ttgcataaaa aacagactac ataatactgt
     2161 aaaacacaac atatgcagtc actatgaatc aactacttag atggtattag tgacctgtag
     2221 aattcgagct ctagagctgc agggcggccg cgatatcccc tatagtgagt cgtattacat
     2281 ggtcatagct gtttcctggc agctctggcc cgtgtctcaa aatctctgat gttacattgc
     2341 acaagataaa aatatatcat catgaacaat aaaactgtct gcttacataa acagtaatac
     2401 aaggggtgtt atgagccata ttcaacggga aacgtcgagg ccgcgattaa attccaacat
     2461 ggatgctgat ttatatgggt ataaatgggc tcgcgataat gtcgggcaat caggtgcgac
     2521 aatctatcgc ttgtatggga agcccgatgc gccagagttg tttctgaaac atggcaaagg
     2581 tagcgttgcc aatgatgtta cagatgagat ggtcagacta aactggctga cggaatttat
     2641 gcctcttccg accatcaagc attttatccg tactcctgat gatgcatggt tactcaccac
     2701 tgcgatcccc ggaaaaacag cattccaggt attagaagaa tatcctgatt caggtgaaaa
     2761 tattgttgat gcgctggcag tgttcctgcg ccggttgcat tcgattcctg tttgtaattg
     2821 tccttttaac agcgatcgcg tatttcgtct cgctcaggcg caatcacgaa tgaataacgg
     2881 tttggttgat gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg aacaagtctg
     2941 gaaagaaatg cataaacttt tgccattctc accggattca gtcgtcactc atggtgattt
     3001 ctcacttgat aaccttattt ttgacgaggg gaaattaata ggttgtattg atgttggacg
     3061 agtcggaatc gcagaccgat accaggatct tgccatccta tggaactgcc tcggtgagtt
     3121 ttctccttca ttacagaaac ggctttttca aaaatatggt attgataatc ctgatatgaa
     3181 taaattgcag tttcatttga tgctcgatga gtttttctaa tcagaattgg ttaattggtt
     3241 gtaacactgg cagagcatta cgctgacttg acgggacggc gcaagctcat gaccaaaatc
     3301 ccttaacgtg agttacgcgt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     3361 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     3421 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     3481 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
     3541 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     3601 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     3661 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     3721 aacgacctac accgaactga gatacctaca gcgtgagcat tgagaaagcg ccacgcttcc
     3781 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     3841 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     3901 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     3961 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgtt
//
