LOCUS       sequence-99061-e         792 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of mitochondria-targeting blue-green emission 
            ratiometric genetically encoded Ca2+ indicator for optical imaging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Zhao Y, Araki S, Wu J, Teramoto T, Chang YF, Nakano M, Abdelfattah 
            AS, Fujiwara M, Ishihara T, Nagai T, Campbell RE
  TITLE     An Expanded Palette of Genetically Encoded Ca2+ Indicators.
  JOURNAL   Science. 2011 Sep 30;333(6051):1888-91. doi: 10.1126/science.1208592
  PUBMED    21903779
REFERENCE   2  (bases 1 to 792)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2011-09-30- 30"; volume: "333"; 
            issue: "6051"; pages: "1888-91"
COMMENT     Sequence Label: CMV-mito-GEM-GECO1
FEATURES             Location/Qualifiers
     source          1..792
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             16..90
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             112..186
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             235..291
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     primer_bind     504..525
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 cctctagact cgagcatgtc tgttctgact cctctgctgc tccggggtct cacaggttcc
       61 gcaagaagac tccccgtgcc tagggccaaa attcattcac tgggggaccc catgagcgtg
      121 ctcaccccac tcctgctgcg ggggctgacc ggcagcgcta ggcggctgcc agtccccagg
      181 gccaagatcc acagtctcgg cgatcccaag gatccaacca tggtcgactc atcacgtcgt
      241 aagtggaata agacaggtca cgcagtcaga gctataggtc ggctgagctc accagagaac
      301 gtgtatataa aggccgacga gcagaagaac ggcatcaagg cgtacttcaa gatccgccac
      361 aacatcgagg gcggcggcgt gcagctcgcc taccactacc agcagatcac ccccataggc
      421 gacggccccg tgctgctgcc cgacaaccac tacctgagcg tgcagtccat actttcgaaa
      481 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
      541 actctcggca tggacgagct gtacaagggt ggcagcggtg gcatggtgag caagggcgag
      601 gagctgttca ccggggtggt gcccatccag gtcgagctgg acggcgacgt aaacggccac
      661 aagttcagcg tgtccggcga gggtgagggc gatgccacct acggcaagct gaccctgaag
      721 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgtcc
      781 tacggcgtgc ag
//
