LOCUS       sequence-99062-e        1061 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mouse NrasG12D was amplified and mutated by PCR from IMAGE clone 
            6475312 (Invitrogen) and cloned downstream from the IRES; 
            subsequently Luciferase was cloned upstream of the IRES.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Zuber J, Radtke I, Pardee TS, Zhao Z, Rappaport AR, Luo W, McCurrach
            ME, Yang MM, Dolan ME, Kogan SC, Downing JR, Lowe SW
  TITLE     Mouse models of human AML accurately predict chemotherapy response.
  JOURNAL   Genes Dev. 2009 Apr 1;23(7):877-89. doi: 10.1101/gad.1771409.
  PUBMED    19339691
REFERENCE   2  (bases 1 to 1061)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1101/gad"; 
            journalName: "Genes Dev"; date: "2009-04-1- 1"; volume: "23"; issue:
            "7"; pages: "877-89"
COMMENT     Sequence Label: MSCV-Luciferase-IRES-NrasG12D
FEATURES             Location/Qualifiers
     source          1..1061
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gcccagaagg taccccattg tatgggatct gatctggggc ctcggtgcac atgctttaca
       61 tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga accacgggga cgtggttttc
      121 ctttgaaaaa cacgatgata atatggccac aaccatgact gagtacaaac tggtggtggt
      181 tggagcagat ggtgttggga aaagcgcctt gacgatccag ctaatccaga accactttgt
      241 ggatgaatat gatcccacca tagaggattc ttaccgaaag caagtggtga ttgatggtga
      301 gacctgcctg ctggacatac tggacacagc tggacaagag gagtacagtg ccatgagaga
      361 ccagtacatg aggacaggcg aagggttcct ctgtgtattt gccatcaata atagcaaatc
      421 atttgcagat attaacctct acagggagca aattaagcgt gtgaaagatt ctgatgatgt
      481 ccccatggtg ctggtaggca acaagtgtga cttgccaaca aggacagttg acacaaagca
      541 agcccacgaa ctggccaaga gttacggaat tccattcatt gagacctcag ccaagacccg
      601 acagggtgtg gaggatgcct tttacacact ggtaagggag atacgccagt accgaatgaa
      661 aaagctcaac agcagtgacg atggcactca aggttgtatg gggctgccct gtgtgctgat
      721 gtagtcgacc tgcagccaag cttatcgata aaataaaaga ttttatttag tctccagaaa
      781 aaggggggaa tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt
      841 tgcaaggcat ggaaaataca taactgagaa tagagaagtt cagatcaagg ttaggaacag
      901 agagacagca gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc
      961 agggccaaga acagatggtc cccagatgcg gtcccgccct cagcagtttc tagagaacca
     1021 tcagatgttt ccagggtgcc ccaaggacct gaaatgaccc t
//
