LOCUS       sequence-99071-e        1009 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mouse NrasG12D was amplified and mutated by PCR from IMAGE clone 
            6475312 (Invitrogen) and cloned downstream from the IRES; 
            subsequently Luciferase was cloned upstream of the IRES.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Zuber J, Radtke I, Pardee TS, Zhao Z, Rappaport AR, Luo W, McCurrach
            ME, Yang MM, Dolan ME, Kogan SC, Downing JR, Lowe SW
  TITLE     Mouse models of human AML accurately predict chemotherapy response.
  JOURNAL   Genes Dev. 2009 Apr 1;23(7):877-89. doi: 10.1101/gad.1771409.
  PUBMED    19339691
REFERENCE   2  (bases 1 to 1009)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1101/gad"; 
            journalName: "Genes Dev"; date: "2009-04-1- 1"; volume: "23"; issue:
            "7"; pages: "877-89"
COMMENT     Sequence Label: MSCV-Luciferase-IRES-NrasG12D
FEATURES             Location/Qualifiers
     source          1..1009
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     8..24
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(122..140)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 atcctccctt tatccagccc tcactccttc tctaggcgcc ggaattagat ccaccatgga
       61 agacgccaaa aacataaaga aaggcccggc gccattctat ccgctggaag atggaaccgc
      121 tggagagcaa ctgcataagg ctatgaagag atacgccctg gttcctggaa caattgcttt
      181 tacagatgca catatcgagg tggacatcac ttacgctgag tacttcgaaa tgtccgttcg
      241 gttggcagaa gctatgaaac gatatgggct gaatacaaat cacagaatcg tcgtatgcag
      301 tgaaaactct cttcaattct ttatgccggt gttgggcgcg ttatttatcg gagttgcagt
      361 tgcgcccgcg aacgacattt ataatgaacg tgaattgctc aacagtatgg gcatttcgca
      421 gcctaccgtg gtgttcgttt ccaaaaaggg gttgcaaaaa attttgaacg tgcaaaaaaa
      481 gctcccaatc atccaaaaaa ttattatcat ggattctaaa acggattacc agggatttca
      541 gtcgatgtac acgttcgtca catctcatct acctcccggt tttaatgaat acgattttgt
      601 gccagagtcc ttcgataggg acaagacaat tgcactgatc atgaactcct ctggatctac
      661 tggtctgcct aaaggtgtcg ctctgcctca tagaactgcc tgcgtgagat tctcgcatgc
      721 cagagatcct atttttggca atcaaatcat tccggatact gcgattttaa gtgttgttcc
      781 attccatcac ggttttggaa tgtttactac actcggatat ttgatatgtg gatttcgagt
      841 cgtcttaatg tatagatttg aagaagagct gtttctgagg agccttcagg attacaagat
      901 tcaaagtgcg ctgctggtgc caaccctatt ctccttcttc gccaaaagca ctctgattga
      961 caaatacgat ttatctaatt tacacgaaat tgcttctggt ggcgctccc
//
