LOCUS       sequence-99074-e         979 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  zf1::yfp fusion with floxed unc-119(+) within an intron of yfp. For 
            creating zf1::yfp knock-ins using unc-119 as a marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 979)
  AUTHORS   Armenti ST, Lohmer LL, Sherwood DR, Nance J
  TITLE     Repurposing an endogenous degradation system for rapid and targeted 
            depletion of C. elegans proteins.
  JOURNAL   Development. 2014 Dec;141(23):4640-7. doi: 10.1242/dev.115048. Epub 
            2014 Nov 5.
  PUBMED    25377555
REFERENCE   2  (bases 1 to 979)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/dev.115048"; journalName: "Development"; date: "2014-12"; 
            volume: "141"; issue: "23"; pages: "4640-7"
COMMENT     Sequence Label: pJN601
FEATURES             Location/Qualifiers
     source          1..979
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(687..707)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 aaaagcaaaa gcaggaaaat taatttcata aactttcact tgattgaaaa attccaaaaa
       61 tactccaaaa ctttttcacg atgagcatga tttgacgtca tgagaggccc agacgtgcga
      121 agaaataaaa attgcggtca taaactgaaa cgtaacatat gataaggtat tttgtgtgcg
      181 gttttttcta tgatgtttca aaaaaattta tcagaagtaa aaaacataag aagtgtacac
      241 agaacattgt gtagattttg ggatagaatt attattaatg gggggaggga gcacaatttt
      301 tttcgacttt ttattacgaa ttaattctat ttttgaatct acacaatttc attgttagag
      361 gtgacttaaa agaagctaaa aaacaaagaa attaagagaa gttttttgat aataacaaaa
      421 atagggggtg ggagcacagg gagaaagagc atgtagggat gttgaagagt aattggactt
      481 agaagtcaga ggcacgggcg cgagatgctg aaaatttaaa tattactcgt tagttagtag
      541 aactcagttt aaacttactc tgatgacagc ggcccctatt atttttgaca ccagacaagt
      601 tggtaatggt agcgaccggc gctcagttgg aattctacga atgctatttg tatagttcat
      661 ccatgccatg tgtaatccca gcagctgtta caaactcaag aaggaccatg tggtctctct
      721 tttcgttggg atctttcgaa agggcagatt ggtaggacag gtaatggttg tctggtaaaa
      781 ggacagggcc atcgccaatt ggagtatttt gttgataatg gtctgctagt tgaacgcttc
      841 catcttcaat gttgtgtcta attttgaagt tctgaaaatt taaatcagat tagttagtta
      901 gtaaaatcat gtttaaactt acaactttga ttccattctt ttgtttgtct gccatgatgt
      961 atacattgtg tgagttata
//
