LOCUS       sequence-99075-e         797 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  zf1::yfp fusion with floxed unc-119(+) within an intron of yfp. For 
            creating zf1::yfp knock-ins using unc-119 as a marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 797)
  AUTHORS   Armenti ST, Lohmer LL, Sherwood DR, Nance J
  TITLE     Repurposing an endogenous degradation system for rapid and targeted 
            depletion of C. elegans proteins.
  JOURNAL   Development. 2014 Dec;141(23):4640-7. doi: 10.1242/dev.115048. Epub 
            2014 Nov 5.
  PUBMED    25377555
REFERENCE   2  (bases 1 to 797)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/dev.115048"; journalName: "Development"; date: "2014-12"; 
            volume: "141"; issue: "23"; pages: "4640-7"
COMMENT     Sequence Label: pJN601
FEATURES             Location/Qualifiers
     source          1..797
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(385..413)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     protein_bind    753..786
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 tttaaacatg atcttactaa ctaactattc tcatttaaat tttcagagct taaaaatggc
       61 tgaaatcact cacaacgatg gatacgctaa caacttggaa atgaaataag cttgcatgcc
      121 tgcaggtcga ctctagagga tccccgggat tggccaaagg acccaaaggt atgtttcgaa
      181 tgatactaac ataacataga acattttcag gaggaccctt ggagggtacc gacagaatac
      241 aaaacgcgac tttgtgatgc gttccgccgt gaaggatact gcccgtacaa cgacaattgc
      301 acatatgctc acggacaaga tgagctgaga gttccgaggg taccggtaga aaaaatgagt
      361 aaaggagaag aacttttcac tggagttgtc ccaattcttg ttgaattaga tggtgatgtt
      421 aatgggcaca aattttctgt cagtggagag ggtgaaggtg atgcaacata cggaaaactt
      481 acccttaaat ttatttgcac tactggaaaa ctacctgttc catgggtaag tttaaacata
      541 tatatactaa ctaaccctga ttatttaaat tttcagccaa cacttgtcac tactttcggt
      601 tatggtcttc aatgcttcgc cagataccca gatcatatga aacagcatga ctttttcaag
      661 agtgccatgc ccgaaggtta tgtacaggaa agaactatat ttttcaaaga tgacgggaac
      721 tacaagacac gtaagtttaa acagttcggt acataacttc gtataatgta tgctatacga
      781 agttatgcct agttcta
//
