LOCUS       sequence-99076-e         838 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a mitochondrial matrix-targeted photoactivable GFP that 
            enables detection and quantification of organelle fusion in living 
            cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Karbowski M, Arnoult D, Chen H, Chan DC, Smith CL, Youle RJ
  TITLE     Quantitation of mitochondrial dynamics by photolabeling of 
            individual organelles shows that mitochondrial fusion is blocked 
            during the Bax activation phase of apoptosis.
  JOURNAL   J Cell Biol. 2004 Feb 16. 164(4):493-9.
  PUBMED    14769861
REFERENCE   2  (bases 1 to 838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2004 Feb 16. 164(4):493-9."
COMMENT     Sequence Label: mito-PAGFP
FEATURES             Location/Qualifiers
     source          1..838
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      14..23
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(51..125)
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     promoter        complement(154..357)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(183..203)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        358..661
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tcgcccttgc tcaccatggt ggcgaccggt ggatccccca acgaatggat cttggcgcgc
       61 ggcactggga gccgccgggc cgagcctgtc aagccccgca gcagcagcgg cgtcaggacg
      121 gacatgctag cggatctgac ggttcactaa accagctctg cttatataga cctcccaccg
      181 tacacgccta ccgcccattt gcgtcaatgg ggcggagttg ttacgacatt ttggaaagtc
      241 ccgttgattt tggtgccaaa acaaactccc attgacgtca atggggtgga gacttggaaa
      301 tccccgtgag tcaaaccgct atccacgccc attgatgtac tgccaaaacc gcatcaccat
      361 ggtaatagcg atgactaata cgtagatgta ctgccaagta ggaaagtccc ataaggtcat
      421 gtactgggca taatgccagg cgggccattt accgtcattg acgtcaatag ggggcgtact
      481 tggcatatga tacacttgat gtactgccaa gtgggcagtt taccgtaaat actccaccca
      541 ttgacgtcaa tggaaagtcc ctattggcgt tactatggga acatacgtca ttattgacgt
      601 caatgggcgg gggtcgttgg gcggtcagcc aggcgggcca tttaccgtaa gttatgtaac
      661 gcggaactcc atatatgggc tatgaactaa tgaccccgta attgattact attaataact
      721 aatgcatggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
      781 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgttttt
//
