LOCUS       sequence-99077-e         692 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd gen lentiviral Gateway destination vector, expression/RNAi, eGFP
            co-expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 692)
  AUTHORS   Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, 
            Campisi J, Yaswen P, Cooper PK, Kaufman PD.
  TITLE     A versatile viral system for expression and depletion of proteins in
            mammalian cells.
  JOURNAL   PLoS One. 2009 Aug 6;4(8):e6529.
  PUBMED    19657394
REFERENCE   2  (bases 1 to 692)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-08-6"; volume: "4(8)"; pages: "e6529"
COMMENT     Sequence Label: pLenti CMV GFP DEST (736-1)
FEATURES             Location/Qualifiers
     source          1..692
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(86..107)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     435..454
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 aaattttgta atccagaggt tgattgtcga cgcggccgct ttacttgtac agctcgtcca
       61 tgccgagagt gatcccggcg gcggtcacga actccagcag gaccatgtga tcgcgcttct
      121 cgttggggtc tttgctcagg gcggactggg tgctcaggta gtggttgtcg ggcagcagca
      181 cggggccgtc gccgatgggg gtgttctgct ggtagtggtc ggcgagctgc acgctgccgt
      241 cctcgatgtt gtggcggatc ttgaagttca ccttgatgcc gttcttctgc ttgtcggcca
      301 tgatatagac gttgtggctg ttgtagttgt actccagctt gtgccccagg atgttgccgt
      361 cctccttgaa gtcgatgccc ttcagctcga tgcggttcac cagggtgtcg ccctcgaact
      421 tcacctcggc gcgggtcttg tagttgccgt cgtccttgaa gaagatggtg cgctcctgga
      481 cgtagccttc gggcatggcg gacttgaaga agtcgtgctg cttcatgtgg tcggggtagc
      541 ggctgaagca ctgcacgccg taggtcaggg tggtcacgag ggtgggccag ggcacgggca
      601 gcttgccggt ggtgcagatg aacttcaggg tcagcttgcc gtaggtggca tcgccctcgc
      661 cctcgccgga cacgctgaac ttgtggccgt tt
//
