LOCUS       sequence-99079-e         769 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd gen lentiviral Gateway destination vector, expression/RNAi, eGFP
            co-expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 769)
  AUTHORS   Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, 
            Campisi J, Yaswen P, Cooper PK, Kaufman PD.
  TITLE     A versatile viral system for expression and depletion of proteins in
            mammalian cells.
  JOURNAL   PLoS One. 2009 Aug 6;4(8):e6529.
  PUBMED    19657394
REFERENCE   2  (bases 1 to 769)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-08-6"; volume: "4(8)"; pages: "e6529"
COMMENT     Sequence Label: pLenti CMV GFP DEST (736-1)
FEATURES             Location/Qualifiers
     source          1..769
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    166..287
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(177..196)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     231..250
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             359..592
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     protein_bind    699..723
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 aaagcctcct cactacttct ggaatagctc agaggccgag gcggcctcgg cctctgcata
       61 aataaaaaaa attagtcagc catggggcgg agaatgggcg gaactgggcg gagttagggg
      121 cgggatgggc ggagttaggg gcgggatagc tagagccaga catgataaga tacattgatg
      181 agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg
      241 atgctattgc tttatttgta accattataa gctgcaataa acaagttcct ctcactctct
      301 gatattcatt tctttgcaag ttataaatac tgaataataa gatgacatga actactactg
      361 ctagagattt tccacactga ctaaaagggt ctgagggatc tctagttacc agagtcacac
      421 aacagacggg cacacactac ttgaagcact caaggcaagc tttattgagg cttaagcagt
      481 gggttcccta gttagccaga gagctcccag gctcagatct ggtctaacca gagagaccca
      541 gtacaagcaa aaagcagatc ttgtcttcgt tgggagtgag ctagcccttc cagtcccccc
      601 ttttctttta aaaagtggct aagatctaca gctgccttgt aagtcattgg tcttaaaggt
      661 accgggccct cgagcggccg ccactgtgct ggatatcaac cactttgtac aagaaagctg
      721 aacgagaaac gtaaaatgat ataaatatca atatattaaa ttagatttt
//
