LOCUS       sequence-99094-e         797 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Yeast codon-optimized yellow fluorescent protein (yEVenus) with PEST
            degron driven by SIC1 promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 797)
  AUTHORS   Mazo-Vargas A, Park H, Aydin M, Buchler NE
  TITLE     Measuring fast gene dynamics in single cells with time-lapse 
            luminescence microscopy
  JOURNAL   Mol Biol Cell. 2014 Sep 17. pii: mbc.E14-07-1187.
  PUBMED    25232010
REFERENCE   2  (bases 1 to 797)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2014 Sep 17. pii: mbc.E14-07-1187."
COMMENT     Sequence Label: pNB813
FEATURES             Location/Qualifiers
     source          1..797
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(385..405)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(385..403)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(424..440)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(424..440)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(437..459)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    448..464
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(472..478,479..496,497..502))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    517..538
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(747..766)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tttgtgacca ttaacatcac catctaattc aaccaaaatt gggacaacac cagtgaataa
       61 ttcttcacct ttagacatgt taattaacgt gtaatagtcc ctgcttcaag ggtcaaaatt
      121 ccgtagaggt ttggttgatt gatttacaaa gacaatacct tatcctttgg atatgccttc
      181 tctcttttta taaatatcaa aagttgcgac gcgaacaaca agccagccat tgaaattttc
      241 agtgtgctgg ctataataga caggatcgac gctgtcgatg gagaaggaaa cacggcaata
      301 gtgcagatat tatcgtgact taatcatatt gatttgaggg gggaatggta tgtttgatcg
      361 ccggaggagc tccagctttt gttcccttta gtgagggtta attgcgcgct tggcgtaatc
      421 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
      481 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
      541 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tggcgtaata
      601 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatgga
      661 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
      721 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
      781 gttcgccggc tttcccc
//
