LOCUS       sequence-99103-e         941 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Firefly luciferase (Promega) driven by MET17 promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 941)
  AUTHORS   Mazo-Vargas A, Park H, Aydin M, Buchler NE
  TITLE     Measuring fast gene dynamics in single cells with time-lapse 
            luminescence microscopy
  JOURNAL   Mol Biol Cell. 2014 Sep 17. pii: mbc.E14-07-1187.
  PUBMED    25232010
REFERENCE   2  (bases 1 to 941)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2014 Sep 17. pii: mbc.E14-07-1187."
COMMENT     Sequence Label: pNB649
FEATURES             Location/Qualifiers
     source          1..941
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     22..38
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     22..38
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(121..139)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 ttctattacc cccatccata ctctagaact agtggatccc ccgggttaat taacatggaa
       61 gacgtcaaaa acataaagaa aggcccggcg ccattctatc cgctggaaga tggaaccgct
      121 ggagagcaac tgcataaggc tatgaagaga tacgccctgg ttcctggaac aattgctttt
      181 acagatgcac atatcgaggt ggacatcact tacgctgagt acttcgaaat gtccgttcgg
      241 ttggcagaag ctatgaaacg atatgggctg aatacaaatc acagaatcgt cgtatgcagt
      301 gaaaactctc ttcaattctt tatgccggtg ttgggcgcgt tatttatcgg agttgcagtt
      361 gcgcccgcga acgacattta taatgaacgt gaattgctca acagtatggg catttcgcag
      421 cctaccgtgg tgttcgtttc caaaaagggg ttgcaaaaaa ttttgaacgt gcaaaaaaag
      481 ctcccaatca tccaaaaaat tattatcatg gattctaaaa cggattacca gggatttcag
      541 tcgatgtaca cgttcgtcac atctcatcta cctcccggtt ttaatgaata cgattttgtg
      601 ccagagtcct tcgataggga caagacaatt gcactgatca tgaactcctc tggatctact
      661 ggtctgccta aaggtgtcgc tctgcctcat agaactgcct gcgtgagatt ctcgcatgcc
      721 agagatccta tttttggcaa tcaaatcatt ccggatactg cgattttaag tgttgttcca
      781 ttccatcacg gttttggaat gtttactaca ctcggatatt tgatatgtgg atttcgagtc
      841 gtcttaatgt atagatttga agaagagctg tttctgagga gccttcagga ttacaagatt
      901 caaagtgcgc tgctggtgcc aaccctattc tccttcttcg c
//
