LOCUS       sequence-99116-e        9265 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Allows the integration of GFP hBub1 in the genome and Tet-inducible 
            expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9265)
  AUTHORS   Perera D, Taylor SS
  TITLE     Sgo1 establishes the centromeric cohesion protection mechanism in G2
            before subsequent Bub1-dependent recruitment in mitosis.
  JOURNAL   J Cell Sci. 2010 Mar 1;123(Pt 5):653-9. doi: 10.1242/jcs.059501. 
            Epub 2010 Feb 2.
  PUBMED    20124418
REFERENCE   2  (bases 1 to 9265)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2010-03-1"; volume: "123(Pt 5):653-9. doi"; pages: " 
            10.1242/jcs.059501. Epub 2010 Feb 2"
COMMENT     Sequence Label: pcDNA5 FRT TO GFP  hBub1 WT
FEATURES             Location/Qualifiers
     source          1..9265
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(1084..1086,1087..1089,1090..1800)
                     /codon_start=1
                     /product="Emerald GFP"
                     /label=EmGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1129..1150)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1390..1409)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1737..1758
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(5115..5139)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(5217..5234)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5223..5447
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5580..5599)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    5731..5778
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             5786..6808
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             5786..6808
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    6938..7059
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6975..6994)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7029..7048
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7108..7124)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7108..7124)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7121..7143)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7132..7148
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7156..7162,7163..7180,7181..7186))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7201..7222
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7339..7356)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7510..8098)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7590..7609)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8269..9060,9061..9129))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8892..8911
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9130..9234)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttggta ccccggggaa ttcggcagat cccgaggtcc gacagcgccc
      961 ggcccagatc cccacgcctg ccaggagcaa gccgagagcc agccggccgg cgcactccga
     1021 ctccgagcag tctctgtcct tcgacccgag ccccgcgccc tttccgggac ccctgccccg
     1081 cggatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg
     1141 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc
     1201 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc
     1261 accctcgtga ccaccttgac ctacggcgtg cagtgcttcg cccgctaccc cgaccacatg
     1321 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc
     1381 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc
     1441 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg
     1501 cacaagctgg agtacaacta caacagccac aaggtctata tcaccgccga caagcagaag
     1561 aacggcatca aggtgaactt caagacccgc cacaacatcg aggacggcag cgtgcagctc
     1621 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac
     1681 cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg
     1741 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag
     1801 ctcgagggat ccgacacccc ggaaaatgtc cttcagatgc ttgaagccca catgcagagc
     1861 tacaagggca atgaccctct tggtgaatgg gaaagataca tacagtgggt agaagagaat
     1921 tttcctgaga ataaagaata cttgataact ttactagaac atttaatgaa ggaattttta
     1981 gataagaaga aataccacaa tgacccaaga ttcatcagtt attgtttaaa atttgctgag
     2041 tacaacagtg acctccatca attttttgag tttctgtaca accatgggat tggaaccctg
     2101 tcatcccctc tgtacattgc ctgggcgggg catctggaag cccaaggaga gctgcagcat
     2161 gccagtgctg tccttcagag aggaattcaa aaccaggctg aacccagaga gttcctgcaa
     2221 caacaataca ggttatttca gacacgcctc actgaaaccc atttgccagc tcaagctaga
     2281 acctcagaac ctctgcataa tgttcaggtt ttaaatcaaa tgataacatc aaaatcaaat
     2341 ccaggaaata acatggcctg catttctaag aatcagggtt cagagctttc tggagtgata
     2401 tcttcagctt gtgataaaga gtcaaatatg gaacgaagag tgatcacgat ttctaaatca
     2461 gaatattctg tgcactcatc tttggcatcc aaagttgatg ttgagcaggt tgttatgtat
     2521 tgcaaggaga agcttattcg tggggaatca gaattttcct ttgaagaatt gagagcccag
     2581 aaatacaatc aacggagaaa gcatgagcaa tgggtaaatg aagacagaca ttatatgaaa
     2641 aggaaagaag caaatgcttt tgaagaacag ctattaaaac agaaaatgga tgaacttcat
     2701 aagaagttgc atcaggtggt ggagacatcc catgaggatc tgcccgcttc ccaggaaagg
     2761 tccgaggtta atccagcacg tatggggcca agtgtaggct cccagcagga actgagagcg
     2821 ccatgtcttc cagtaaccta tcagcagaca ccagtgaaca tggaaaagaa cccaagagag
     2881 gcacctcctg ttgttcctcc tttggcaaat gctatttctg cagctttggt gtccccagcc
     2941 accagccaga gcattgctcc tcctgttcct ttgaaagccc agacagtaac agactccatg
     3001 tttgcagtgg ccagcaaaga tgctggatgt gtgaataaga gtactcatga attcaagcca
     3061 cagagtggag cagagatcaa agaagggtgt gaaacacata aggttgccaa cacaagttct
     3121 tttcacacaa ctccaaacac atcactggga atggttcagg caacgccatc caaagtgcag
     3181 ccatcaccca ccgtgcacac aaaagaagca ttaggtttca tcatgaatat gtttcaggct
     3241 cctacacttc ctgatatttc tgatgacaaa gatgaatggc aatctctaga tcaaaatgaa
     3301 gatgcatttg aagcccagtt tcaaaaaaat gtaaggtcat ctggggcttg gggagtcaat
     3361 aagatcatct cttctttgtc atctgctttt catgtgtttg aagatggaaa caaagaaaat
     3421 tatggattac cacagcctaa aaataaaccc acaggagcca ggacctttgg agaacgctct
     3481 gtcagcagac ttccttcaaa accaaaggag gaagtgcctc atgctgaaga gtttttggat
     3541 gactcaactg tatggggtat tcgctgcaac aaaaccctgg cacccagtcc taagagccca
     3601 ggagacttca catctgctgc acaacttgcg tctacaccat tccacaagct tccagtggag
     3661 tcagtgcaca ttttagaaga taaagaaaat gtggtagcaa aacagtgtac ccaggcgact
     3721 ttggattctt gtgaggaaaa catggtggtg ccttcaaggg atggaaaatt cagtccaatt
     3781 caagagaaaa gcccaaaaca ggccttgtcg tctcacatgt attcagcatc cttacttcgt
     3841 ctgagccagc ctgctgcagg tggggtactt acctgtgagg cagagttggg cgttgaggct
     3901 tgcagactca cagacactga cgctgccatt gcagaagatc caccagatgc tattgctggg
     3961 ctccaagcag aatggatgca gatgagttca cttgggactg ttgatgctcc aaacttcatt
     4021 gttgggaacc catgggatga taagctgatt ttcaaacttt tatctgggct ttctaaacca
     4081 gtgagttcct atccaaatac ttttgaatgg caatgtaaac ttccagccat caagcccaag
     4141 actgaatttc aattgggttc taagctggtc tatgtccatc accttcttgg agaaggagcc
     4201 tttgcccagg tgtacgaagc tacccaggga gatctgaatg atgctaaaaa taaacagaaa
     4261 tttgttttaa aggtccaaaa gcctgccaac ccctgggaat tctacattgg gacccagttg
     4321 atggaaagac taaagccatc tatgcagcac atgtttatga agttctattc tgcccactta
     4381 ttccagaatg gcagtgtatt agtaggagag ctctacagct atggaacatt attaaatgcc
     4441 attaacctct ataaaaatac ccctgaaaaa gtgatgcctc aaggtcttgt catctctttt
     4501 gctatgagaa tgctttacat gattgagcaa gtgcatgact gtgaaatcat tcatggagac
     4561 attaaaccag acaatttcat acttggaaac ggatttttgg aacaggatga tgaagatgat
     4621 ttatctgctg gcttggcact gattgacctg ggtcagagta tagatatgaa actttttcca
     4681 aaaggaacta tattcacagc aaagtgtgaa acatctggtt ttcagtgtgt tgagatgctc
     4741 agcaacaaac catggaacta ccagatcgat tactttgggg ttgctgcaac agtatattgc
     4801 atgctctttg gcacttacat gaaagtgaaa aatgaaggag gagagtgtaa gcctgaaggt
     4861 ctttttagaa ggcttcctca tttggatatg tggaatgaat tttttcatgt tatgttgaat
     4921 attccagatt gtcatcatct tccatctttg gatttgttaa ggcaaaagct gaagaaagta
     4981 tttcaacaac actatactaa caagattagg gccctacgta ataggctaat tgtactgctc
     5041 ttagaatgta agcgttcacg aaaataaaat ttggatatag ccagtcctta aaaatccccc
     5101 tgtaaatatg aatctcccaa ctttcttgta caaagttggc attataagaa agcattgctt
     5161 atcaatttgt tgcaacgaac taagcggccg cgggcccgtt taaacccgct gatcagcctc
     5221 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
     5281 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
     5341 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
     5401 ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga
     5461 aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc
     5521 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     5581 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     5641 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     5701 acttgattag ggtgatggtt cacgtaccta gaagttccta ttccgaagtt cctattctct
     5761 agaaagtata ggaacttcct tggccaaaaa gcctgaactc accgcgacgt ctgtcgagaa
     5821 gtttctgatc gaaaagttcg acagcgtctc cgacctgatg cagctctcgg agggcgaaga
     5881 atctcgtgct ttcagcttcg atgtaggagg gcgtggatat gtcctgcggg taaatagctg
     5941 cgccgatggt ttctacaaag atcgttatgt ttatcggcac tttgcatcgg ccgcgctccc
     6001 gattccggaa gtgcttgaca ttggggaatt cagcgagagc ctgacctatt gcatctcccg
     6061 ccgtgcacag ggtgtcacgt tgcaagacct gcctgaaacc gaactgcccg ctgttctgca
     6121 gccggtcgcg gaggccatgg atgcgatcgc tgcggccgat cttagccaga cgagcgggtt
     6181 cggcccattc ggaccgcaag gaatcggtca atacactaca tggcgtgatt tcatatgcgc
     6241 gattgctgat ccccatgtgt atcactggca aactgtgatg gacgacaccg tcagtgcgtc
     6301 cgtcgcgcag gctctcgatg agctgatgct ttgggccgag gactgccccg aagtccggca
     6361 cctcgtgcac gcggatttcg gctccaacaa tgtcctgacg gacaatggcc gcataacagc
     6421 ggtcattgac tggagcgagg cgatgttcgg ggattcccaa tacgaggtcg ccaacatctt
     6481 cttctggagg ccgtggttgg cttgtatgga gcagcagacg cgctacttcg agcggaggca
     6541 tccggagctt gcaggatcgc cgcggctccg ggcgtatatg ctccgcattg gtcttgacca
     6601 actctatcag agcttggttg acggcaattt cgatgatgca gcttgggcgc agggtcgatg
     6661 cgacgcaatc gtccgatccg gagccgggac tgtcgggcgt acacaaatcg cccgcagaag
     6721 cgcggccgtc tggaccgatg gctgtgtaga agtactcgcc gatagtggaa accgacgccc
     6781 cagcactcgt ccgagggcaa aggaatagca cgtactacga gatttcgatt ccaccgccgc
     6841 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca
     6901 gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa
     6961 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     7021 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac
     7081 ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     7141 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     7201 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     7261 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     7321 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     7381 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     7441 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     7501 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     7561 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     7621 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     7681 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     7741 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     7801 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     7861 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     7921 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
     7981 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     8041 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     8101 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     8161 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     8221 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     8281 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     8341 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     8401 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     8461 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     8521 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     8581 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     8641 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     8701 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     8761 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     8821 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     8881 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     8941 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     9001 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     9061 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     9121 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     9181 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     9241 tccccgaaaa gtgccacctg acgtc
//
