LOCUS       sequence-99123-e       11469 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Allows the integration of myc Separase delta 55/S1126A in the genome
            and Tet-inducible expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11469)
  AUTHORS   Holland AJ, Bottger F, Stemmann O, Taylor SS
  TITLE     Protein phosphatase 2A and separase form a complex regulated by 
            separase autocleavage.
  JOURNAL   J Biol Chem. 2007 Aug 24;282(34):24623-32. Epub 2007 Jun 29.
  PUBMED    17604273
REFERENCE   2  (bases 1 to 11469)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2007-08-24"; volume: "282(34)"; pages: "24623-32. 
            Epub 2007 Jun 29"
COMMENT     Sequence Label: pcDNA5 FRT TO myc Separase delta 55/S1126A
FEATURES             Location/Qualifiers
     source          1..11469
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    820..838
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    841..859
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     869..893
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             1154..1183
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(7421..7438)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7427..7651
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7784..7803)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    7935..7982
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             7990..9012
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             7990..9012
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    9142..9263
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9179..9198)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9233..9252
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9312..9328)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9312..9328)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9325..9347)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9336..9352
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9360..9366,9367..9384,9385..9390))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9405..9426
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9543..9560)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9714..10302)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9794..9813)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10473..11264,11265..11333))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11096..11115
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11334..11438)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
      841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
      901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
      961 ctctagcgtt taaacttaag cttggtaccc cggggaattc ggcagatccc gaggtccgac
     1021 agcgcccggc ccagatcccc acgcctgcca ggagcaagcc gagagccagc cggccggcgc
     1081 actccgactc cgagcagtct ctgtccttcg acccgagccc cgcgcccttt ccgggacccc
     1141 tgccccgcgg atggagcaga agctcatctc agaagaagac ctcgagagga gcttcaaaag
     1201 agtcaacttt gggactctgc taagcagcca gaaggaggct gaagagttgc tgcccgactt
     1261 gaaggagttc ctgtccaacc ctccagctgg ttttcccagc agccgatctg atgctgagag
     1321 gagacaagct tgtgatgcca tcctgagggc ttgcaaccag cagctgactg ctaagctagc
     1381 ttgccctagg catctgggga gcctgctgga gctggcagag ctggcctgtg atggctactt
     1441 agtgtctacc ccacagcgtc ctcccctcta cctggaacga attctctttg tcttactgcg
     1501 gaatgctgct gcacaaggaa gcccagaggc cacactccgc cttgctcagc ccctccatgc
     1561 ctgcttggtg cagtgctctc gcgaggctgc tccccaggac tatgaggccg tggctcgggg
     1621 cagcttttct ctgctttgga agggggcaga agccctgttg gaacggcgag ctgcatttgc
     1681 agctcggctg aaggccttga gcttcctagt actcttggag gatgaaagta ccccttgtga
     1741 ggttcctcac tttgcttctc caacagcctg tcgagcggta gctgcccatc agctatttga
     1801 tgccagtggc catggtctaa atgaagcaga tgctgatttc ctagatgacc tgctctccag
     1861 gcacgtgatc agagccttgg tgggtgagag agggagctct tctgggcttc tttctcccca
     1921 gagggccctc tgcctcttgg agctcacctt ggaacactgc cgtcgctttt gctggagccg
     1981 ccaccatgac aaagccatca gcgcagtgga gaaggctcac agttacctaa ggaacaccaa
     2041 tctagcccct agccttcagc tatgtcagct gggggttaag ctgctgcagg ttggggagga
     2101 aggacctcag gcagtggcca agcttctgat caaggcatca gctgtcctga gcaagagtat
     2161 ggaggcacca tcacccccac ttcgggcatt gtatgagagc tgccagttct tcctttcagg
     2221 cctggaacga ggcaccaaga ggcgctatag acttgatgcc attctgagcc tctttgcttt
     2281 tcttggaggg tactgctctc ttctgcagca gctgcgggat gatggtgtgt atgggggctc
     2341 ctccaagcaa cagcagtctt ttcttcagat gtactttcag ggacttcacc tctacactgt
     2401 ggtggtttat gactttgccc aaggctgtca gatagttgat ttggctgacc tgacccaact
     2461 agtggacagt tgtaaatcta ccgttgtctg gatgctggag gccttagagg gcctgtcggg
     2521 ccaagagctg acggaccaca tggggatgac cgcttcttac accagtaatt tggcctacag
     2581 cttctatagt cacaagctct atgccgaggc ctgtgccatc tctgagccgc tctgtcagca
     2641 cctgggtttg gtgaagccag gcacttatcc cgaggtgcct cctgagaagt tgcacaggtg
     2701 cttccggcta caagtagaga gtttgaagaa actgggtaaa caggcccagg gctgcaagat
     2761 ggtgattttg tggctggcag ccctgcaacc ctgtagccct gaacacatgg ctgagccagt
     2821 cactttctgg gttcgggtca agatggatgc ggccagggct ggagacaagg agctacagct
     2881 aaagactctg cgagacagcc tcagtggctg ggacccggag accctggccc tcctgctgag
     2941 ggaggagctg caggcctaca aggcggtgcg ggccgacact ggacaggaac gcttcaacat
     3001 catctgtgac ctcctggagc tgagccccga ggagacacca gccggggcct gggcacgagc
     3061 cacccacctg gtagaactgg ctcaggtgct ctgctaccac gactttacgc agcagaccaa
     3121 ctgctctgct ctggatgcta tccgggaagc cctgcagctt ctggactctg tgaggcctga
     3181 ggcccaggcc agagatcagc ttctggacga taaagcacag gccttgctgt ggctttacat
     3241 ctgtactctg gaagccaaaa tacaggaagg tatcgagcgg gatcggagag cccaggcccc
     3301 tggtaacttg gaggaatttg aagtcaatga cctgaactat gaagataaac tccaggaaga
     3361 tcgtttccta tacagtaaca ttgccttcaa cctggctgca gatgctgctc agtccaaatg
     3421 cctggaccaa gccctggccc tgtggaagga gctgcttaca aaggggcagg ccccagctgt
     3481 acggtgtctc cagcagacag cagcctcact gcagatccta gcagccctct accagctggt
     3541 ggcaaagccc atgcaggctc tggaggtcct cctgctgcta cggattgtct ctgagagact
     3601 gaaggaccac tcgaaggcag ctggctcctc ctgccacatc acccagctcc tcctgaccct
     3661 cggctgtccc agctatgccc agttacacct ggaagaggca gcatcgagcc tgaagcatct
     3721 cgatcagact actgacacat acctgctcct ttccctgacc tgtgatctgc ttcgaagtca
     3781 actctactgg actcaccaga aggtgaccaa gggtgtctct ctgctgctgt ctgtgcttcg
     3841 ggatcctgcc ctccagaagt cctccaaggc ttggtacttg ctgcgtgtcc aggtcctgca
     3901 gctggtggca gcttacctta gcctcccgtc aaacaacctc tcacactccc tgtgggagca
     3961 gctctgtgcc caaggctggc agacacctga gatagctctc atagactccc ataagctcct
     4021 ccgaagcatc atcctcctgc tgatgggcag tgacattctc tcaactcaga aagcagctgt
     4081 ggagacatcg tttttggact atggtgaaaa tctggtacaa aaatggcagg ttctttcaga
     4141 ggtgctgagc tgctcagaga agctggtctg ccacctgggc cgcctgggta gtgtgagtga
     4201 agccaaggcc ttttgcttgg aggccctaaa acttacaaca aagctgcaga taccacgcca
     4261 gtgtgccctg ttcctggtgc tgaagggcga gctggagctg gcccgcaatg acattgatct
     4321 ctgtcagtcg gacctgcagc aggttctgtt cttgcttgag tcttgcacag agtttggtgg
     4381 ggtgactcag cacctggact ctgtgaagaa ggtccacctg cagaagggga agcagcaggc
     4441 ccaggtcccc tgtcctccac agctcccaga ggaggagctc ttcctaagag gccctgctct
     4501 agagctggtg gccactgtgg ccaaggagcc tggccccata gcaccttcta caaactccgc
     4561 cccagtcttg aaaaccaagc cccagcccat acccaacttc ctgtcccatt cacccacctg
     4621 tgactgctcg ctctgcgcca gccctgtcct cacagcagtc tgtctgcgct gggtattggt
     4681 cacggcaggg gtgaggctgg ccatgggcca ccaagcccag ggtctggatc tgctgcaggt
     4741 cgtgctgaag ggctgtcctg aagccgctga gcgcctcacc caagctctcc aagcttccct
     4801 gaatcataaa acacccccct ccttggttcc aagcctcttg gatgagatct tggctcaagc
     4861 atacacactg ttggcactgg agggcctgaa ccagccatca aacgagagcc tgcagaaggt
     4921 tctacagtca gggctgaagt ttgtagcagc acggataccc cacctagagc cctggcgagc
     4981 cagcctgctc ttgatttggg ccctcacaaa actaggtggc ctcagctgct gtactaccca
     5041 actttttgca agctcctggg gctggcagcc accattaata aaaagtgtcc ctggctcaga
     5101 gccctctaag actcagggcc aaaaacgttc tggacgaggg cgccaaaagt tagcctctgc
     5161 tcccctgagc ctcaataata cctctcagaa aggtctggaa ggtagaggac tgccctgcac
     5221 acctaaaccc ccagaccgga tcaggcaagc tggccctcat gtccccttca cggtgtttga
     5281 ggaagtctgc cctacagaga gcaagcctga agtaccccag gcccccaggg tacaacagag
     5341 agtccagacg cgcctcaagg tgaacttcag tgatgacagt gacttggaag accctgtctc
     5401 agctgaggcc tggctggcag aggagcctaa gagacggacc ggtgccagtg accaggccag
     5461 gcctggccct gagatcatga ggaccatccc tgaggaagaa ctgactgaca actggagaaa
     5521 aatgagcttt gagatcctca ggggctctga cggggaagac tcagcctcag gtgggaagac
     5581 tccagctccg ggccctgagg cagcttctgg agaatgggag ctgctgaggc tggattccag
     5641 caagaagaag ctgcccagcc catgcccaga caaggagagt gacaaggacc ttggtcctcg
     5701 gctccagctc ccctcagccc ccgtagccac tggtctttct accctggact ccatctgtga
     5761 ctccctgagt gttgctttcc ggggcattag tcactgtcct cctagtgggc tctatgccca
     5821 cctctgccgc ttcctggcct tgtgcctggg ccaccgggat ccttatgcca ctgctttcct
     5881 tgtcaccgag tctgtctcca tcacctgtcg ccaccagctg ctcacccacc tccacagaca
     5941 gctcagcaag gcccagaagc accgaggatc acttgaaata gcagaccagc tgcaggggct
     6001 gagccttcag gagatgcctg gagatgtccc cctggcccgc atccagcgcc tcttttcctt
     6061 cagggctttg gaatctggcc acttccccca gcctgaaaag gagagtttcc aggagcgcct
     6121 ggctctgatc cccagtgggg tgactgtgtg tgtgttggcc ctggccaccc tccagcccgg
     6181 aaccgtgggc aacaccctcc tgctgacccg gctggaaaag gacagtcccc cagtcagtgt
     6241 gcagattccc actggccaga acaagcttca tctgcgttca gtcctgaatg agtttgatgc
     6301 catccagaag gcacagaaag agaacagcag ctgtactgac aagcgagaat ggtggacagg
     6361 gcggctggca ctggaccaca ggatggaggt tctcatcgct tccctagaga agtctgtgct
     6421 gggctgctgg aaggggctgc tgctgccgtc cagtgaggag cccggccctg cccaggaggc
     6481 ctcccgccta caggagctgc tacaggactg tggctggaaa tatcctgacc gcactctgct
     6541 gaaaatcatg ctcagtggtg ccggtgccct cacccctcag gacattcagg ccctggccta
     6601 cgggctgtgc ccaacccagc cagagcgagc ccaggagctc ctgaatgagg cagtaggacg
     6661 tctacagggc ctgacagtac caagcaatag ccaccttgtc ttggtcctag acaaggactt
     6721 gcagaagctg ccgtgggaaa gcatgcccag cctccaagca ctgcctgtca cccggctgcc
     6781 ctccttccgc ttcctactca gctactccat catcaaagag tatggggcct cgccagtgct
     6841 gagtcaaggg gtggatccac gaagtacctt ctatgtcctg aaccctcaca ataacctgtc
     6901 aagcacagag gagcaatttc gagccaattt cagcagtgaa gctggctgga gaggagtggt
     6961 tggggaggtg ccaagacctg aacaggtgca ggaagccctg acaaagcatg atttgtatat
     7021 ctatgcaggg catggggctg gtgcccgctt ccttgatggg caggctgtcc tgcggctgag
     7081 ctgtcgggca gtggccctgc tgtttggctg tagcagtgcg gccctggctg tgcatggaaa
     7141 cctggagggg gctggcatcg tgctcaagta catcatggct ggttgcccct tgtttctggg
     7201 taatctctgg gatgtgactg accgcgacat tgaccgctac acggaagctc tgctgcaagg
     7261 ctggcttgga gcaggcccag gggcccccct tctctactat gtaaaccagg cccgccaagc
     7321 tccccgactc aagtatctta ttggggctgc acctatagcc tatggcttgc ctgtctctct
     7381 gcggtgagcg gccgcgggcc cgtttaaacc cgctgatcag cctcgactgt gccttctagt
     7441 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
     7501 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat
     7561 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc
     7621 aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc
     7681 tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     7741 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     7801 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     7861 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     7921 ggttcacgta cctagaagtt cctattccga agttcctatt ctctagaaag tataggaact
     7981 tccttggcca aaaagcctga actcaccgcg acgtctgtcg agaagtttct gatcgaaaag
     8041 ttcgacagcg tctccgacct gatgcagctc tcggagggcg aagaatctcg tgctttcagc
     8101 ttcgatgtag gagggcgtgg atatgtcctg cgggtaaata gctgcgccga tggtttctac
     8161 aaagatcgtt atgtttatcg gcactttgca tcggccgcgc tcccgattcc ggaagtgctt
     8221 gacattgggg aattcagcga gagcctgacc tattgcatct cccgccgtgc acagggtgtc
     8281 acgttgcaag acctgcctga aaccgaactg cccgctgttc tgcagccggt cgcggaggcc
     8341 atggatgcga tcgctgcggc cgatcttagc cagacgagcg ggttcggccc attcggaccg
     8401 caaggaatcg gtcaatacac tacatggcgt gatttcatat gcgcgattgc tgatccccat
     8461 gtgtatcact ggcaaactgt gatggacgac accgtcagtg cgtccgtcgc gcaggctctc
     8521 gatgagctga tgctttgggc cgaggactgc cccgaagtcc ggcacctcgt gcacgcggat
     8581 ttcggctcca acaatgtcct gacggacaat ggccgcataa cagcggtcat tgactggagc
     8641 gaggcgatgt tcggggattc ccaatacgag gtcgccaaca tcttcttctg gaggccgtgg
     8701 ttggcttgta tggagcagca gacgcgctac ttcgagcgga ggcatccgga gcttgcagga
     8761 tcgccgcggc tccgggcgta tatgctccgc attggtcttg accaactcta tcagagcttg
     8821 gttgacggca atttcgatga tgcagcttgg gcgcagggtc gatgcgacgc aatcgtccga
     8881 tccggagccg ggactgtcgg gcgtacacaa atcgcccgca gaagcgcggc cgtctggacc
     8941 gatggctgtg tagaagtact cgccgatagt ggaaaccgac gccccagcac tcgtccgagg
     9001 gcaaaggaat agcacgtact acgagatttc gattccaccg ccgccttcta tgaaaggttg
     9061 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg
     9121 ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc
     9181 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     9241 tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg
     9301 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     9361 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     9421 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     9481 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     9541 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     9601 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     9661 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     9721 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     9781 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     9841 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     9901 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     9961 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
    10021 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
    10081 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
    10141 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
    10201 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
    10261 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
    10321 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
    10381 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
    10441 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
    10501 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
    10561 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
    10621 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
    10681 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
    10741 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
    10801 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
    10861 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
    10921 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
    10981 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
    11041 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
    11101 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
    11161 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
    11221 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
    11281 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
    11341 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
    11401 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
    11461 cctgacgtc
//
