LOCUS       sequence-99128-e         884 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 884)
  TITLE     PTEN
REFERENCE   2  (bases 1 to 884)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: 863 pSG5L HA NES PTEN
FEATURES             Location/Qualifiers
     source          1..884
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          64..636
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     644..663
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     691..710
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        691..709
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             743..769
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             743..769
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             749..775
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             782..808
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
ORIGIN
        1 agaggctttt ttggaggcct aggcttttgc aaaaagctcc ggatcgatcc tgagaacttc
       61 agggtgagtt tggggaccct tgattgttct ttctttttcg ctattgtaaa attcatgtta
      121 tatggagggg gcaaagtttt cagggtgttg tttagaatgg gaagatgtcc cttgtatcac
      181 catggaccct catgataatt ttgtttcttt cactttctac tctgttgaca accattgtct
      241 cctcttattt tcttttcatt ttctgtaact ttttcgttaa actttagctt gcatttgtaa
      301 cgaattttta aattcacttt tgtttatttg tcagattgta agtactttct ctaatcactt
      361 ttttttcaag gcaatcaggg tatattatat tgtacttcag cacagtttta gagaacaatt
      421 gttataatta aatgataagg tagaatattt ctgcatataa attctggctg gcgtggaaat
      481 attcttattg gtagaaacaa ctacatcctg gtcatcatcc tgcctttctc tttatggtta
      541 caatgatata cactgtttga gatgaggata aaatactctg agtccaaacc gggcccctct
      601 gctaaccatg ttcatgcctt cttctttttc ctacagctcc tgggcaacgt gctggttatt
      661 gtgctgtctc atcattttgg caaagaattg taatacgact cactataggg cgaattgtcg
      721 acccgggaag cttaccatgg cctaccccta cgacgtgccc gactacgcct ccctcggatc
      781 tctgccgccg ctggagcgcc tgacgctggg gatccatgac agccatcatc aaagagatcg
      841 ttagcagaaa caaaaggaga tatcaagagg atggattcga ctta
//
