LOCUS       sequence-99132-e         483 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Flp-dependent expression of Kir2.1-T2A-tdTomato.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 483)
  AUTHORS   Xue M, Atallah BV, Scanziani M
  TITLE     Equalizing excitation-inhibition ratios across visual cortical 
            neurons.
  JOURNAL   Nature. 2014 Jul 31;511(7511):596-600. doi: 10.1038/nature13321. 
            Epub 2014 Jun 22.
  PUBMED    25043046
REFERENCE   2  (bases 1 to 483)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature13321"; journalName: "Nature"; date: "2014-07-31- 
            31"; volume: "511"; issue: "7511"; pages: "596-600"
COMMENT     Sequence Label: pAAV-EF1-alpha-F-FLEX- Kir2.1-T2A-tdTomato
FEATURES             Location/Qualifiers
     source          1..483
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    92..125
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(218..241)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(416..435)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 aggtaccgaa gttcctattc tatcagaagt ataggaactt cgcagaatgg tagctggatt
       61 gtagctgcta ttagcaatat gaaacctctt agaagttcct attctctaga aagtatagga
      121 acttcgaatt cttacttgta cagctcgtcc atgccgtaca ggaacaggtg gtggcggccc
      181 tcggagcgct cgtactgttc cacgatggtg tagtcctcgt tgtgggaggt gatgtccagc
      241 ttggtgtcca cgtagtagta gccgggcagt tgcacgggct tcttggccat gtagatggtc
      301 ttgaactcca ccaggtagtg gccgccgtcc ttcagcttca gggcctggtg gatctcgccc
      361 ttcagcacgc cgtcgcgggg gtacaggcgc tcggtggagg cctcccagcc catggtcttc
      421 ttctgcatta cggggccgtc gggggggaag ttggtgccgc gcatcttcac cttgtagatc
      481 agc
//
