LOCUS       sequence-99133-e         498 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Flp-dependent expression of mNaChBac-T2A-tdTomato.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 498)
  AUTHORS   Xue M, Atallah BV, Scanziani M
  TITLE     Equalizing excitation-inhibition ratios across visual cortical 
            neurons.
  JOURNAL   Nature. 2014 Jul 31;511(7511):596-600. doi: 10.1038/nature13321. 
            Epub 2014 Jun 22.
  PUBMED    25043046
REFERENCE   2  (bases 1 to 498)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature13321"; journalName: "Nature"; date: "2014-07-31- 
            31"; volume: "511"; issue: "7511"; pages: "596-600"
COMMENT     Sequence Label: pAAV-EF1-alpha-F-FLEX-mNaChBac-T2A-tdTomato
FEATURES             Location/Qualifiers
     source          1..498
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    94..127
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(220..243)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(418..437)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 tgaggtaccg aagttcctat tctatcagaa gtataggaac ttcgcagaat ggtagctgga
       61 ttgtagctgc tattagcaat atgaaacctc ttagaagttc ctattctcta gaaagtatag
      121 gaacttcgaa ttcttacttg tacagctcgt ccatgccgta caggaacagg tggtggcggc
      181 cctcggagcg ctcgtactgt tccacgatgg tgtagtcctc gttgtgggag gtgatgtcca
      241 gcttggtgtc cacgtagtag tagccgggca gttgcacggg cttcttggcc atgtagatgg
      301 tcttgaactc caccaggtag tggccgccgt ccttcagctt cagggcctgg tggatctcgc
      361 ccttcagcac gccgtcgcgg gggtacaggc gctcggtgga ggcctcccag cccatggtct
      421 tcttctgcat tacggggccg tcggggggga agttggtgcc gcgcatcttc accttgtaga
      481 tcagcgtgcc gtcctgca
//
