LOCUS       sequence-99138-e         855 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Co-expresses two green fluorescent protein variants, a short 
            fluorescence lifetime GFP variant in the nucleus and a GFP variant 
            with a long lifetime in the cytoplasm.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Joosen L, Hink MA, Gadella TW Jr, Goedhart J
  TITLE     Effect of fixation procedures on the fluorescence lifetimes of 
            Aequorea victoria derived fluorescent proteins.
  JOURNAL   J Microsc. 2014 Sep 1. doi: 10.1111/jmi.12168.
  PUBMED    25179491
REFERENCE   2  (bases 1 to 855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Microsc. 
            2014 Sep 1. doi: 10.1111/jmi.12168."
COMMENT     Sequence Label: 3xNLS-SGFP2(T65G)-T2A-SGFP2-3xNES
FEATURES             Location/Qualifiers
     source          1..855
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(268..516)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSTQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELY"
     primer_bind     complement(307..328)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     656..675
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt
       61 tttttaaagc aagtaaaacc tctacaaatg tggtatggct gattatgatc tagagtcgcg
      121 gccgctttac ttgtacgagt ccagatccaa ctcttccagc ttgttctgca gctcactgta
      181 cgagtccaga tccaactctt ccagcttgtt ctgcagctca ctgtacgagt ccagatccaa
      241 ctcttccagc ttgttctgca gctcactgta cagctcgtcc atgccgagag tgatcccggc
      301 ggcggtcacg aactccagca ggaccatgtg atcgcgcttc tcgttggggt ctttgctcag
      361 cttggactgg gtgctcaggt agtggttgtc gggcagcagc acggggccgt cgccgatggg
      421 ggtgttctgc tggtagtggt cggcgagctg cacgccgccg tcctcgatgt tgtggcggat
      481 cttgaagttg gccttgatgc cgttcttctg cttgtcggcg gtgatataga cgttgtggct
      541 gttgtagttg tactccagct tgtgccccag gatgttgccg tcctccttga agtcgatgcc
      601 cttcagctcg atgcggttca ccagggtgtc gccctcgaac ttcacctcgg cgcgggtctt
      661 gtagttgccg tcgtccttga agaagatggt gcgctcctgg acgtagcctt cgggcatggc
      721 ggacttgaag aagtcgtgct gcttcatgtg gtcggggtag cgggcgaagc actgcacgcc
      781 gtaggtcagg gtggtcacga gggtgggcca gggcacgggc agcttgccgg tggtgcagat
      841 gaacttcagg gtcag
//
