LOCUS       sequence-99139-e         876 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Co-expresses two cyan fluorescent protein variants, a short 
            fluorescence lifetime CFP variant in the nucleus and a CFP variant 
            with a long lifetime (mTurquoise2) in the cytoplasm.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Joosen L, Hink MA, Gadella TW Jr, Goedhart J
  TITLE     Effect of fixation procedures on the fluorescence lifetimes of 
            Aequorea victoria derived fluorescent proteins.
  JOURNAL   J Microsc. 2014 Sep 1. doi: 10.1111/jmi.12168.
  PUBMED    25179491
REFERENCE   2  (bases 1 to 876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Microsc. 
            2014 Sep 1. doi: 10.1111/jmi.12168."
COMMENT     Sequence Label: pH2B-SCFP1-2A-mTq2-NES
FEATURES             Location/Qualifiers
     source          1..876
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(178..426)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSTQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELY"
     primer_bind     complement(217..238)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     566..585
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     825..846
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt
       61 tttttaaagc aagtaaaacc tctacaaatg tggtatggct gattatgatc tagagtcgcg
      121 gccgctttac ttgtacgagt ccagatccaa ctcttccagc ttgttctgca gctcactgta
      181 cagctcgtcc atgccgagag tgatcccggc ggcggtcacg aactccagca ggaccatgtg
      241 atcgcgcttc tcgttggggt ctttgctcag cttggactgg gtgctcaggt agtggttgtc
      301 gggcagcagc acggggccgt cgccgatggg ggtgttctgc tggtagtggt cggcgagctg
      361 cacgccgccg tcctcgatgt tgtggcggat cttgaagttg gccttgatgc cgttcttctg
      421 cttgtcggcg gtgatataga cgttgtcgct aaagtagttg tactccagct tgtgccccag
      481 gatgttgccg tcctccttga agtcgatgcc cttcagctcg atgcggttca ccagggtgtc
      541 gccctcgaac ttcacctcgg cgcgggtctt gtagttgccg tcgtccttga agaagatggt
      601 gcgctcctgg acgtagcctt cgggcatggc ggacttgaag aagtcgtgct gcttcatgtg
      661 gtcggggtag cgggcgaagc actgcacgcc ccaggacagg gtggtcacga gggtgggcca
      721 gggcacgggc agcttgccgg tggtgcagat gaacttcagg gtcagcttgc cgtaggtggc
      781 atcgccctcg ccctcgccgg acacgctgaa cttgtggccg tttacgtcgc cgtccagctc
      841 gaccaggatg ggcaccaccc cggtgaacag ctcctc
//
