LOCUS       sequence-99141-e         867 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutively expresses AmiE with proK1 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Bienick MS, Young KW, Klesmith JR, Detwiler EE, Tomek KJ, Whitehead 
            TA
  TITLE     The Interrelationship between Promoter Strength, Gene Expression, 
            and Growth Rate.
  JOURNAL   PLoS One. 2014 Oct 6;9(10):e109105. doi: 
            10.1371/journal.pone.0109105. eCollection 2014.
  PUBMED    25286161
REFERENCE   2  (bases 1 to 867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-10-6"; volume: "9(10):e109105. doi"; pages: " 
            10.1371/journal.pone.0109105. eCollection 2014"
COMMENT     Sequence Label: pJK_proK1_amiE
FEATURES             Location/Qualifiers
     source          1..867
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             326..348
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc
       61 tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc
      121 ctcaacgaca ggagcacgat catgcgcacc cgtggggccg ccatgccggc cacagctaac
      181 accacgtcgt ccctatctgc tgccctaggt ctatgagtgg ttgctggata actttactgg
      241 catgcataag gctcgtaata tatattcagg gagaccacaa cggtttccct ctacaaataa
      301 ttttgtttaa ctttctagaa ataattttgt ttaactttaa gaaggagata tacatatgag
      361 acatggcgat attagctcgt caaatgatac cgtaggcgta gccgtggtga attacaagat
      421 gccgcgttta catactgctg ctgaagtcct ggataatgcc cgcaaaattg cggaaatgat
      481 cgttggtatg aagcaaggtc tgccgggcat ggatctggtt gtgtttcctg aatattcttt
      541 acagggtatt atgtacgacc ctgctgaaat gatggaaaca gccgtggcga ttccaggcga
      601 agaaacggaa atctttagcc gtgcttgtag aaaagcaaat gtttggggtg tgttctccct
      661 gaccggcgaa cgtcatgaag aacaccctag aaaggcacca tacaacactc tggtcttgat
      721 cgataacaac ggtgaaatcg tacaaaagta cagaaagatc atcccatggt gtccgattga
      781 aggctggtat ccaggtggcc agacatacgt ctctgaaggt ccgaaaggca tgaagatctc
      841 attaattatc tgcgatgacg gtaatta
//
