LOCUS       sequence-99142-e         757 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid encoding iCas9 and tracrRNA on pRS415 backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 757)
  AUTHORS   Bao Z, Xiao H, Liang J, Zhang L, Xiong X, Sun N, Si T, Zhao H
  TITLE     Homology-Integrated CRISPR-Cas (HI-CRISPR) System for One-Step 
            Multigene Disruption in Saccharomyces cerevisiae.
  JOURNAL   ACS Synth Biol. 2014 Sep 19.
  PUBMED    25207793
REFERENCE   2  (bases 1 to 757)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2014 Sep 19."
COMMENT     Sequence Label: pCT
FEATURES             Location/Qualifiers
     source          1..757
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 aatggatggt actgaggaat tattggtgaa actaaatcgt gaagatttgc tgcgcaagca
       61 acggaccttt gacaacggct ctattaccca tcaaattcac ttgggtgagc tgcatgctat
      121 tttgagaaga caagaagact tttatccatt tttaaaagac aatcgtgaga agattgaaaa
      181 aatcttgact tttcgaattc cttattatgt tggtccattg gcgcgtggca atagtcgttt
      241 tgcatggatg actcggaagt ctgaagaaac aattacccca tggaattttg aagaagttgt
      301 cgataaaggt gcttcagctc aatcatttat tgaacgcatg acaaactttg ataaaaatct
      361 tccaaatgaa aaagtactac caaaacatag tttgctttat gagtatttta cggtttataa
      421 cgaattgaca aaggtcaaat atgttactga aggaatgcga aaaccagcat ttctttcagg
      481 tgaacagaag aaagccattg ttgatttact cttcaaaaca aatcgaaaag taaccgttaa
      541 gcaattaaaa gaagattatt tcaaaaaaat agaatgtttt gatagtgttg aaatttcagg
      601 agttgaagat agatttaatg cttcattagg tacctaccat gatttgctaa aaattattaa
      661 agataaagat tttttggata atgaagaaaa tgaagatatc ttagaggata ttgttttaac
      721 attgacctta tttgaagata gggagatgat tgaggaa
//
