LOCUS       sequence-99143-e         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Zhang Z, Xu J, Koppetsch BS, Wang J, Tipping C, Ma S, Weng Z, 
            Theurkauf WE, Zamore PD
  TITLE     Heterotypic piRNA Ping-Pong requires qin, a protein with both E3 
            ligase and Tudor domains.
  JOURNAL   Mol Cell. 2011 Nov 18;44(4):572-84. doi: 
            10.1016/j.molcel.2011.10.011.
  PUBMED    22099305
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2011.10"; journalName: "Mol Cell"; date: 
            "2011-11-18- 18"; volume: "44"; issue: "4"; pages: "572-84"
COMMENT     Sequence Label: pUASp-Flag-Myc-Qin-Tudor-attB
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      246..255
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             255..320
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     regulatory      336..345
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             345..374
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             384..413
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             423..452
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             462..491
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             501..530
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             564..593
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    606..630
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tttttttttg aaaccggtga tagagcctga accagaaaag ataaaagaag gctataccag
       61 ctgggagtac acaaacagag taagtttgaa tagtaaaaaa aatcatttat gtaaacaata
      121 acgtgactgt gcgttaggtc ctgttcattg atcgaggcct gtctagagaa gcttgttcga
      181 atctcgagtg cgcgcttccg gaggtataca cctaggcggt accactgcag tgaattcgga
      241 gctccgccac catggactac aaagaccatg acggtgatta taaagatcat gacatcgatt
      301 acaaggatga cgatgacaag caccggttga gctccgccac catggagcaa aagctcattt
      361 ctgaagagga cttgaatgaa atggagcaaa agctcatttc tgaagaggac ttgaatgaaa
      421 tggagcaaaa gctcatttct gaagaggact tgaatgaaat ggagcaaaag ctcatttctg
      481 aagaggactt gaatgaaatg gagcaaaagc tcatttctga agaggacttg aatgaaatgg
      541 agagcttggg cgacctcacc atggagcaaa agctcatttc tgaagaggac ttgaatcacc
      601 ggtatacaag tttgtacaaa aaagcaggct tcgtggttca acgggccaaa caaaaactaa
      661 acgactatgc caccgaaacg ttggccagca tttcgaagcg attttcccac ctgcacggcc
      721 ttctccaggt ggccgagctg caggtcattg agaagctgcg cgagtccagt ttgccaccac
      781 aattgaaact caacgaagcc atgggccagc taagtggcta cgaaactgtc atcgagcgat
      841 taacaaaaga gtttaaacag
//
