LOCUS       sequence-99144-e         930 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the cyan fluorescent protein variant mTurquoise.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Goedhart J, van Weeren L, Hink MA, Vischer NO, Jalink K, Gadella TW 
            Jr
  TITLE     Bright cyan fluorescent protein variants identified by fluorescence 
            lifetime screening.
  JOURNAL   Nat Methods. 2010 Feb;7(2):137-9. doi: 10.1038/nmeth.1415. Epub 2010
            Jan 17.
  PUBMED    20081836
REFERENCE   2  (bases 1 to 930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1415"; journalName: "Nat Methods"; date: "2010-02"; 
            volume: "7"; issue: "2"; pages: "137-9"
COMMENT     Sequence Label: pmTurquoise-C1
FEATURES             Location/Qualifiers
     source          1..930
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    122..187
                     /label=MCS
                     /note="multiple cloning site"
     CDS             complement(join(188..898,899..901,902..904))
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2010)"
                     /label=mTurquoise
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(230..251)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     579..598
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     838..859
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      901..910
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta
       61 aagcaagtaa aacctctaca aatgtggtat ggctgattat gatcagttat ctagatccgg
      121 tggatcccgg gcccgcggta ccgtcgactg cagaattcga agcttgagct cgagatctga
      181 gtccggactt gtacagctcg tccatgccga gagtgatccc ggcggcggtc acgaactcca
      241 gcaggaccat gtgatcgcgc ttctcgttgg ggtctttgct cagcttggac tgggtgctca
      301 ggtagtggtt gtcgggcagc agcacggggc cgtcgccgat gggggtgttc tgctggtagt
      361 ggtcggcgag ctgcacgccg ccgtcctcga tgttgtggcg gatcttgaag ttggccttga
      421 tgccgttctt ctgcttgtcg gcggtgatat agacgttgtc gctgatgtag ttgtactcca
      481 gcttgtgccc caggatgttg ccgtcctcct tgaagtcgat gcccttcagc tcgatgcggt
      541 tcaccagggt gtcgccctcg aacttcacct cggcgcgggt cttgtagttg ccgtcgtcct
      601 tgaagaagat ggtgcgctcc tggacgtagc cttcgggcat ggcggacttg aagaagtcgt
      661 gctgcttcat gtggtcgggg tagcgggcga agcactgcac gccccaggac agggtggtca
      721 cgagggtggg ccagggcacg ggcagcttgc cggtggtgca gatgaacttc agggtcagct
      781 tgccgtaggt ggcatcgccc tcgccctcgc cggacacgct gaacttgtgg ccgtttacgt
      841 cgccgtccag ctcgaccagg atgggcacca ccccggtgaa cagctcctcg cccttgctca
      901 ccatggtggc gaccggtagc gctagcggat
//
