LOCUS       sequence-99145-e         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the cyan fluorescent protein variant mTurquoise.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Goedhart J, van Weeren L, Hink MA, Vischer NO, Jalink K, Gadella TW 
            Jr
  TITLE     Bright cyan fluorescent protein variants identified by fluorescence 
            lifetime screening.
  JOURNAL   Nat Methods. 2010 Feb;7(2):137-9. doi: 10.1038/nmeth.1415. Epub 2010
            Jan 17.
  PUBMED    20081836
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1415"; journalName: "Nat Methods"; date: "2010-02"; 
            volume: "7"; issue: "2"; pages: "137-9"
COMMENT     Sequence Label: pmTurquoise-N1
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(122..835,836..838,839..841))
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2010)"
                     /label=mTurquoise
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(167..188)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     516..535
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     775..796
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      838..847
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt
       61 aaagcaagta aaacctctac aaatgtggta tggctgatta tgatctagag tcgcggccgc
      121 tttacttgta cagctcgtcc atgccgagag tgatcccggc ggcggtcacg aactccagca
      181 ggaccatgtg atcgcgcttc tcgttggggt ctttgctcag cttggactgg gtgctcaggt
      241 agtggttgtc gggcagcagc acggggccgt cgccgatggg ggtgttctgc tggtagtggt
      301 cggcgagctg cacgccgccg tcctcgatgt tgtggcggat cttgaagttg gccttgatgc
      361 cgttcttctg cttgtcggcg gtgatataga cgttgtcgct gatgtagttg tactccagct
      421 tgtgccccag gatgttgccg tcctccttga agtcgatgcc cttcagctcg atgcggttca
      481 ccagggtgtc gccctcgaac ttcacctcgg cgcgggtctt gtagttgccg tcgtccttga
      541 agaagatggt gcgctcctgg acgtagcctt cgggcatggc ggacttgaag aagtcgtgct
      601 gcttcatgtg gtcggggtag cgggcgaagc actgcacgcc ccaggacagg gtggtcacga
      661 gggtgggcca gggcacgggc agcttgccgg tggtgcagat gaacttcagg gtcagcttgc
      721 cgtaggtggc atcgccctcg ccctcgccgg acacgctgaa cttgtggccg tttacgtcgc
      781 cgtccagctc gaccaggatg ggcaccaccc cggtgaacag ctcctcgccc ttgctcacca
      841 tggtggcgac cggtggatcc cgggcccgcg gtaccgtcga
//
