LOCUS       sequence-99146-e         829 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the Fluorescence Lifetime Imaging Microscopy 
            (FLIM)-compatible PKA activity sensor FLIM-AKAR in a Cre dependent 
            manner in an AAV backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Chen Y, Saulnier JL, Yellen G, Sabatini BL
  TITLE     A PKA activity sensor for quantitative analysis of endogenous GPCR 
            signaling via 2-photon FRET-FLIM imaging.
  JOURNAL   Front Pharmacol. 2014 Apr 2;5:56. doi: 10.3389/fphar.2014.00056. 
            eCollection 2014.
  PUBMED    24765076
REFERENCE   2  (bases 1 to 829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Pharmacol."; date: "2014-04-2"; volume: "5:56. doi"; pages: " 
            10.3389/fphar.2014.00056. eCollection 2014"
COMMENT     Sequence Label: AAV-FLEX-FLIM-AKAR
FEATURES             Location/Qualifiers
     source          1..829
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
       61 ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc
      121 atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctggctgat
      181 cattaccagc agaacacacc tatcggagac gggcccgtcc tgctccctga taatcattat
      241 ctgtctactc agagtaagct ctcaaaagac ccaaacgaga aacgggatca catggtgctg
      301 ctcgagttcg tcactgccgc tcgcatgcat aagttttccc aggagcagat cggtgaaaat
      361 atcgtgtgcc gggtcatctg tacaactgga cagatcccca ttcgcgacct gtctgccgac
      421 atcagtcagg tgctgaagga gaaaaggtct attaagaaag tctggacatt cggaagaaac
      481 cccgcttgcg actatcacct ggggaacatt agcaggctgt ccaataagca ctttcagatt
      541 ctcctgggcg aagacgggaa cctcctcctc aacgacatca gcactaacgg gacctggtta
      601 aatgggcaaa aagtcgagaa gaacagcaat cagttactgt ctcaaggtga tgaaataacc
      661 gttggtgtag gcgtggaatc agatatttta tctctggtca ttttcataaa cgacaaattt
      721 aagcagtgcc tcgagcagaa caaagttgat cgctctgcag gtaagccagg cagcggcgag
      781 ggcagcacca agggcctgcg tcgcgccacc ctggtggacg gcggcaccg
//
