LOCUS       sequence-99147-e         836 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the non-phosphorylatable point mutant (T391A) of FLIM-AKAR
            in a Cre dependent manner in an AAV backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Chen Y, Saulnier JL, Yellen G, Sabatini BL
  TITLE     A PKA activity sensor for quantitative analysis of endogenous GPCR 
            signaling via 2-photon FRET-FLIM imaging.
  JOURNAL   Front Pharmacol. 2014 Apr 2;5:56. doi: 10.3389/fphar.2014.00056. 
            eCollection 2014.
  PUBMED    24765076
REFERENCE   2  (bases 1 to 836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Pharmacol."; date: "2014-04-2"; volume: "5:56. doi"; pages: " 
            10.3389/fphar.2014.00056. eCollection 2014"
COMMENT     Sequence Label: AAV-FLEX-FLIM-AKART391A
FEATURES             Location/Qualifiers
     source          1..836
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc
       61 tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca
      121 tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctggctgatc
      181 attaccagca gaacacacct atcggagacg ggcccgtcct gctccctgat aatcattatc
      241 tgtctactca gagtaagctc tcaaaagacc caaacgagaa acgggatcac atggtgctgc
      301 tcgagttcgt cactgccgct cgcatgcata agttttccca ggagcagatc ggtgaaaata
      361 tcgtgtgccg ggtcatctgt acaactggac agatccccat tcgcgacctg tctgccgaca
      421 tcagtcaggt gctgaaggag aaaaggtcta ttaagaaagt ctggacattc ggaagaaacc
      481 ccgcttgcga ctatcacctg gggaacatta gcaggctgtc caataagcac tttcagattc
      541 tcctgggcga agacgggaac ctcctcctca acgacatcag cactaacggg acctggttaa
      601 atgggcaaaa agtcgagaag aacagcaatc agttactgtc tcaaggtgat gaaataaccg
      661 ttggtgtagg cgtggaatca gatattttat ctctggtcat tttcataaac gacaaattta
      721 agcagtgcct cgagcagaac aaagttgatc gctctgcagg taagccaggc agcggcgagg
      781 gcagcaccaa gggcctgcgt cgcgccgccc tggtggacgg cggcaccggc ggctca
//
