LOCUS       sequence-99148-e         815 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  crRNA expression plasmid on pRS423 backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Bao Z, Xiao H, Liang J, Zhang L, Xiong X, Sun N, Si T, Zhao H
  TITLE     Homology-Integrated CRISPR-Cas (HI-CRISPR) System for One-Step 
            Multigene Disruption in Saccharomyces cerevisiae.
  JOURNAL   ACS Synth Biol. 2014 Sep 19.
  PUBMED    25207793
REFERENCE   2  (bases 1 to 815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2014 Sep 19."
COMMENT     Sequence Label: pCR
FEATURES             Location/Qualifiers
     source          1..815
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(9..28)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10..28)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(38..55)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(38..54)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(47..69)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        complement(258..526)
                     /label=SNR52 promoter
                     /note="promoter for the S. cerevisiae small nucleolar RNA 
                     gene SNR52"
     primer_bind     602..623
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc
       61 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg
      121 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
      181 tgaatggcga atgggacgcg ccctgtagcg gctgaggtct ctttgggacc attcaaaaca
      241 gcatagctct aaaacccgat catttatctt tcactgcgga gaagtttcga acgccgaaac
      301 atgcgcacca actttcactt ctacagcgtt tgaccaaaat cttttgaaca gaacattgta
      361 gggtgtgaaa aaatgcgcac ctttaccgct agcccaagag ggcactacaa aatctagagt
      421 tgtacttcaa acgtacatgt aatcaccttg tatatactcg aaagaaaaca tcaagtttct
      481 gtataaatat gagtgaaagc ataatcatac attatctttt caaagactcg agcacgtagt
      541 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
      601 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
      661 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
      721 tttaacgcga attttaacaa aatattaacg cttacaattt cctgatgcgg tattttctcc
      781 ttacgcatct gtgcggtatt tcacaccgca tagat
//
