LOCUS       sequence-99149-e         868 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutively expresses AmiE with proB promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Bienick MS, Young KW, Klesmith JR, Detwiler EE, Tomek KJ, Whitehead 
            TA
  TITLE     The Interrelationship between Promoter Strength, Gene Expression, 
            and Growth Rate.
  JOURNAL   PLoS One. 2014 Oct 6;9(10):e109105. doi: 
            10.1371/journal.pone.0109105. eCollection 2014.
  PUBMED    25286161
REFERENCE   2  (bases 1 to 868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-10-6"; volume: "9(10):e109105. doi"; pages: " 
            10.1371/journal.pone.0109105. eCollection 2014"
COMMENT     Sequence Label: pJK_proB_amiE
FEATURES             Location/Qualifiers
     source          1..868
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             322..344
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc
       61 gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca
      121 acgacaggag cacgatcatg cgcacccgtg gggccgccat gccggccaca gctaacacca
      181 cgtcgtccct atctgctgcc ctaggtctat gagtggttgc tggataactt tacgggcatg
      241 cataaggctc gtaatatata ttcagggaga ccacaacggt ttccctctac aaataatttt
      301 gtttaacttt ctagaaataa ttttgtttaa ctttaagaag gagatataca tatgagacat
      361 ggcgatatta gctcgtcaaa tgataccgta ggcgtagccg tggtgaatta caagatgccg
      421 cgtttacata ctgctgctga agtcctggat aatgcccgca aaattgcgga aatgatcgtt
      481 ggtatgaagc aaggtctgcc gggcatggat ctggttgtgt ttcctgaata ttctttacag
      541 ggtattatgt acgaccctgc tgaaatgatg gaaacagccg tggcgattcc aggcgaagaa
      601 acggaaatct ttagccgtgc ttgtagaaaa gcaaatgttt ggggtgtgtt ctccctgacc
      661 ggcgaacgtc atgaagaaca ccctagaaag gcaccataca acactctggt cttgatcgat
      721 aacaacggtg aaatcgtaca aaagtacaga aagatcatcc catggtgtcc gattgaaggc
      781 tggtatccag gtggccagac atacgtctct gaaggtccga aaggcatgaa gatctcatta
      841 attatctgcg atgacggtaa ttatccgg
//
