LOCUS       sequence-99153-e        1009 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Construct used for viral production of virus expressing the TVA 
            receptor, Rabies protein G and a marker protein TagBFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Betley JN, Cao ZF, Ritola KD, Sternson SM
  TITLE     Parallel, redundant circuit organization for homeostatic control of 
            feeding behavior.
  JOURNAL   Cell. 2013 Dec 5;155(6):1337-50. doi: 10.1016/j.cell.2013.11.002.
  PUBMED    24315102
REFERENCE   2  (bases 1 to 1009)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.11"; journalName: "Cell"; date: "2013-12-5- 5";
            volume: "155"; issue: "6"; pages: "1337-50"
COMMENT     Sequence Label: AAV-FLEX-rev-BFP-2a-TVA-2a-RabiesG
FEATURES             Location/Qualifiers
     source          1..1009
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(53..86)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(97..130)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
ORIGIN
        1 ttgatttgat accgcgggcc ccgcgtggac ggtacccttc aggtcgacgt cgataacttc
       61 gtatagcata cattatacga agttatccga atcgcaataa cttcgtatag gatactttat
      121 acgaagttat ttggcgcgcc actagttcac aagcgcgttt cacctcctga cttgtgtgac
      181 tcccacgacg aaatgatctt cccggactgc ggagtgacgg acacttctct ccccgtaccc
      241 ctcaagttgt gctgtgtggg ttccgatctg ttcacccttc tacagcacgt catgaggaag
      301 atgatgagca tgagtgccgt caacgcgcct gcgctcaaca ggacgtactt tccccaattc
      361 ggcaaaccga ggtccacccc ggacacttgg ttatgtacgt ccgggaggtg gacttccaca
      421 aaatcttccg cttcatctcc gtccttaaag acggtggaag ggtcggccaa cgggtgcacg
      481 agcggaatta ctgagctttc gagcaactcc atgtgttgct gaaggaggga tgactgcatt
      541 tcgggaatca ggacattccc atcgggaccg aggataattc cattgaaaaa cactccattg
      601 acatgggggt ggcaccgtcc tccgactctc agacaacctt ttgagggaag aatctcgttc
      661 cacgtccgta ccgacttata gtgcgcgtcg gcttccataa gggttttgtt aaagatggtg
      721 tatgcctttc cgaacccagg gacaagtttg cggaggtggc tcagccggcg aaaggacacc
      781 gacttcgtag tcataattga ttccagcgcg tccagacact cttccctttt tcgtacaagt
      841 tcctcgacga caaggtgttc gatttcgtct gatctaaagt catgaaggtt cacgagcttg
      901 tcgggtgggc accacttcgt ttcgttggac gtctgcatcg acacccacgt tccatccata
      961 agcctaagtc cgaggacccc acacagtttc agtttgcacg cccccttga
//
