LOCUS       sequence-99154-e         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutively expresses AmiE with proB promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Bienick MS, Young KW, Klesmith JR, Detwiler EE, Tomek KJ, Whitehead 
            TA
  TITLE     The Interrelationship between Promoter Strength, Gene Expression, 
            and Growth Rate.
  JOURNAL   PLoS One. 2014 Oct 6;9(10):e109105. doi: 
            10.1371/journal.pone.0109105. eCollection 2014.
  PUBMED    25286161
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-10-6"; volume: "9(10):e109105. doi"; pages: " 
            10.1371/journal.pone.0109105. eCollection 2014"
COMMENT     Sequence Label: pJK_proB_amiE
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(15..32)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gcagccggat ctcagtggtg gtggtggtgg tgctcgaggc cttcatacgg cagacgacct
       61 actgggcatt gggcgacacc tgttgtggag cgagtcaggc gttccacgtt ttctcttgcc
      121 ttttcagcat cagtaaccca ggtacggtag aattcaaatg ggcattctgc cagaccacgg
      181 tcgccatcgc ccgaagcctg caaaccggag taacctctgt gcaagatttt aaacaaatgg
      241 ttctgacttt ggtcattcgc acgggcatcg cggatttgtg acagagacaa ctgcgcgtat
      301 tgaatgccca tttcttcttc gccgcattca cccagagtac ggccgtcaaa accgataatg
      361 gcactatgac cgaagtagct ataaacgcca tcaaaacctg cagcgtttgc tacagcgaca
      421 taacagttat tcgcccaggc cattgcctta gccatcataa cttgttggtc tttagcaggg
      481 tacatatagc cttggcagcg aacgatcaat tccgcaccct tcatggcaca atctctccaa
      541 atttccggat aattaccgtc atcgcagata attaatgaga tcttcatgcc tttcggacct
      601 tcagagacgt atgtctggcc acctggatac cagccttcaa tcggacacca tgggatgatc
      661 tttctgtact tttgtacgat ttcaccgttg ttatcgatca agaccagagt gttgtatggt
      721 gcctttctag ggtgttcttc atgacgttcg ccggtcaggg agaacacacc ccaaacattt
      781 gcttttctac aagcacggct aaagatttcc gtttcttcgc ctggaatcgc cacggctgtt
      841 tccatcattt cagcagggtc gtacataata ccctgtaaag
//
