LOCUS       sequence-99155-e         887 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutively expresses PAD (phenolic acid decarboxylase).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Tomek KJ, Saldarriaga CR, Velasquez FP, Liu T, Hodge DB, Whitehead 
            TA
  TITLE     Removal and upgrading of lignocellulosic fermentation inhibitors by 
            in situ biocatalysis and liquid-liquid extraction.
  JOURNAL   Biotechnol Bioeng. 2015 Mar;112(3):627-32. doi: 10.1002/bit.25473. 
            Epub 2014 Nov 24.
  PUBMED    25311910
REFERENCE   2  (bases 1 to 887)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/bit.25473";
            journalName: "Biotechnol Bioeng"; date: "2015-03"; volume: "112"; 
            issue: "3"; pages: "627-32"
COMMENT     Sequence Label: pJK_kanR_proJK1_PAD
FEATURES             Location/Qualifiers
     source          1..887
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             326..348
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             851..868
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc
       61 tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc
      121 ctcaacgaca ggagcacgat catgcgcacc cgtggggccg ccatgccggc cacagctaac
      181 accacgtcgt ccctatctgc tgccctaggt ctatgagtgg ttgctggata actttatggg
      241 catgcataag gctcgtataa tatattcagg gagaccacaa cggtttccct ctacaaataa
      301 ttttgtttaa ctttctagaa ataattttgt ttaactttaa gaaggagata tacatatggg
      361 catggaaaac tttatcggct cacacatgat ctacacttac gaaaacggtt gggaatacga
      421 aatctacatc aaaaacgacc acaccattga ttaccgtatc catagcggta tggtcgctgg
      481 ccgttgggtt cgtgaccaag aagtcaacat cgtaaagctg acagaaggtg tttacaaggt
      541 gtcttggacg gaaccgaccg gcactgatgt ctcattgaac ttcatgccta acgaaaaacg
      601 catgcatggt attatctttt tccctaagtg ggttcatgaa cacccagaaa ttaccgtgtg
      661 ttaccagaac gatcacatcg acttgatgaa agaatctcgt gaaaagtatg aaacctaccc
      721 aaaatatgtt gtgccggaat ttgcagaaat cactttcctg aagaatgaag gcgttgacaa
      781 cgaagaagtg atttcctacg ccccatacga aggcatgaca gatgacatta gagcaggtcg
      841 cttactcgag caccaccacc accaccactg agatccggct gctaaca
//
