LOCUS       sequence-99156-e         934 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Construct used for viral production of virus expressing the TVA 
            receptor, Rabies protein G and a marker protein TagBFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 934)
  AUTHORS   Betley JN, Cao ZF, Ritola KD, Sternson SM
  TITLE     Parallel, redundant circuit organization for homeostatic control of 
            feeding behavior.
  JOURNAL   Cell. 2013 Dec 5;155(6):1337-50. doi: 10.1016/j.cell.2013.11.002.
  PUBMED    24315102
REFERENCE   2  (bases 1 to 934)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.11"; journalName: "Cell"; date: "2013-12-5- 5";
            volume: "155"; issue: "6"; pages: "1337-50"
COMMENT     Sequence Label: AAV-FLEX-rev-BFP-2a-TVA-2a-RabiesG
FEATURES             Location/Qualifiers
     source          1..934
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(48..81)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(92..125)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      152..161
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             875..898
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             902..925
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 aattttgtaa tccagaggtt gattatcgat aagcttgatg atcttcgata acttcgtata
       61 ggatacttta tacgaagtta ttgcgattcg gataacttcg tatagcatac attatacgaa
      121 gttatcaagg ccggccggat ccactagtgc cgccaccatg gtcagcaaag gagaggagtt
      181 gatcaaggaa aacatgcata tgaaactgta tatggaggga acagtggata accatcattt
      241 caagtgtacg tcagaagggg agggaaagcc gtacgagggg acacagacca tgcggatcaa
      301 agtagtggaa ggaggcccat tgccctttgc cttcgacatt ctggcaacct cgttccttta
      361 tggatcgaaa acgtttatca atcacacgca ggggattcct gatttcttta agcagtcatt
      421 tcccgaaggg tttacgtggg agagagtgac gacgtatgag gatggtggag tgctgactgc
      481 gacacaagac acgtcgctcc aagacgggtg ccttatctac aacgtgaaga tcagaggagt
      541 caatttcaca tccaatggtc cggtcatgca gaaaaagaca ctcggatggg aggcattcac
      601 tgaaaccctg tacccggctg acggtggtct ggaagggagg aatgatatgg cgctcaaatt
      661 ggtagggggt tcgcatctta ttgcgaacgc caagaccaca tataggagca aaaagcctgc
      721 caagaatctc aagatgcccg gagtgtacta tgtcgactac cgacttgaac gcatcaaaga
      781 ggcgaacaac gagacttacg tcgagcagca cgaagtcgcg gtggctcggt attgtgacct
      841 tccctccaag ctcggccaca aactcaacgg tggggattac aaggacgacg acgacaaagg
      901 cgactacaag gatgatgatg acaaggggga ctac
//
