LOCUS       sequence-99160-e         657 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Split-GFP assay in plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 657)
  AUTHORS   Kim JG, Li X, Roden JA, Taylor KW, Aakre CD, Su B, Lalonde S, Kirik 
            A, Chen Y, Baranage G, McLane H, Martin GB, Mudgett MB
  TITLE     Xanthomonas T3S Effector XopN Suppresses PAMP-Triggered Immunity and
            Interacts with a Tomato Atypical Receptor-Like Kinase and TFT1.
  JOURNAL   Plant Cell. 2009 Apr;21(4):1305-23. doi: 10.1105/tpc.108.063123. 
            Epub 2009 Apr 14.
  PUBMED    19366901
REFERENCE   2  (bases 1 to 657)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1105/tpc.108.063123"; journalName: "Plant Cell"; date: 
            "2009-04"; volume: "21"; issue: "4"; pages: "1305-23"
COMMENT     Sequence Label: PXCGW
FEATURES             Location/Qualifiers
     source          1..657
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(113..237)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     463..484
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             536..553
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 agagtggctg atctcagcca ccgcgaaaat gacatcaaaa acgccattaa cctgatgttc
       61 tggggaatat aaatgtcagg ctcccttata cacagccagt ctgcaggtcg accatagtga
      121 ctggatatgt tgtgttttac agtattatgt agtctgtttt ttatgcaaaa tctaatttaa
      181 tatattgata tttatatcat tttacgtttc tcgttcagct ttcttgtaca aagtggtgaa
      241 gcttggtgga gctggtggag ctggtggagc tgccgacaag cagaagaacg gcatcaaggc
      301 caacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca
      361 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac
      421 ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt
      481 cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagggtg gagctcatca
      541 tcatcatcat cattaatcta gagtccgcaa aaatcaccag tctctctcta caaatctatc
      601 tctctctatt tttctccaga ataatgtgtg agtagttccc agataaggga attaggg
//
