LOCUS       sequence-99164-e         941 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  lentiviral expression of v-H-Ras and luciferase/EGFP reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 941)
  AUTHORS   Holczbauer A, Factor VM, Andersen JB, Marquardt JU, Kleiner DE, 
            Raggi C, Kitade M, Seo D, Akita H, Durkin ME, Thorgeirsson SS
  TITLE     Modeling pathogenesis of primary liver cancer in lineage-specific 
            mouse cell types.
  JOURNAL   Gastroenterology. 2013 Jul;145(1):221-31. doi: 
            10.1053/j.gastro.2013.03.013. Epub 2013 Mar 19.
  PUBMED    23523670
REFERENCE   2  (bases 1 to 941)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1053/j.gastro.2013.03.013"; journalName: "Gastroenterology"; 
            date: "2013-07"; volume: "145"; issue: "1"; pages: "221-31"
COMMENT     Sequence Label: pSico-CAG-v-H-Ras-IRES-Luciferase/EGFP
FEATURES             Location/Qualifiers
     source          1..941
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(109..125)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(110..126)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     CDS             complement(join(131..844,845..847,848..850))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(134..844,845..847,848..850))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(176..197)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     525..544
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     784..805
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      847..856
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atctttcaca aattttgtaa tccagaggtt gattatcgat aagcttgata tcgaattgta
       61 cctagtggaa ccggaaccct taaacatgat ctcgtgaagc gagcttatcg ataccgtcga
      121 cctcgagtta ctacttgtac agctcgtcca tgccgagagt gatcccggcg gcggtcacga
      181 actccagcag gaccatgtga tcgcgcttct cgttggggtc tttgctcagg gcggactggg
      241 tgctcaggta gtggttgtcg ggcagcagca cggggccgtc gccgatgggg gtgttctgct
      301 ggtagtggtc ggcgagctgc acgctgccgt cctcgatgtt gtggcggatc ttgaagttca
      361 ccttgatgcc gttcttctgc ttgtcggcca tgatatagac gttgtggctg ttgtagttgt
      421 actccagctt gtgccccagg atgttgccgt cctccttgaa gtcgatgccc ttcagctcga
      481 tgcggttcac cagggtgtcg ccctcgaact tcacctcggc gcgggtcttg tagttgccgt
      541 cgtccttgaa gaagatggtg cgctcctgga cgtagccttc gggcatggcg gacttgaaga
      601 agtcgtgctg cttcatgtgg tcggggtagc ggctgaagca ctgcacgccg taggtcaggg
      661 tggtcacgag ggtgggccag ggcacgggca gcttgccggt ggtgcagatg aacttcaggg
      721 tcagcttgcc gtaggtggca tcgccctcgc cctcgccgga cacgctgaac ttgtggccgt
      781 ttacgtcgcc gtccagctcg accaggatgg gcaccacccc ggtgaacagc tcctcgccct
      841 tgctcaccat ggtggcgacc ggtggatccc gcacggcgat cttgccgccc ttcttggcct
      901 taatgagaat ctcgcggatc ttgcgggcgt ccaacttgcc g
//
