LOCUS       sequence-99166-e         550 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  lentiviral expression of v-H-Ras and luciferase/EGFP reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 550)
  AUTHORS   Holczbauer A, Factor VM, Andersen JB, Marquardt JU, Kleiner DE, 
            Raggi C, Kitade M, Seo D, Akita H, Durkin ME, Thorgeirsson SS
  TITLE     Modeling pathogenesis of primary liver cancer in lineage-specific 
            mouse cell types.
  JOURNAL   Gastroenterology. 2013 Jul;145(1):221-31. doi: 
            10.1053/j.gastro.2013.03.013. Epub 2013 Mar 19.
  PUBMED    23523670
REFERENCE   2  (bases 1 to 550)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1053/j.gastro.2013.03.013"; journalName: "Gastroenterology"; 
            date: "2013-07"; volume: "145"; issue: "1"; pages: "221-31"
COMMENT     Sequence Label: pSico-CAG-v-H-Ras-IRES-Luciferase/EGFP
FEATURES             Location/Qualifiers
     source          1..550
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    55..172
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    242..262
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 ggatcggcac tgcgtgcgcc aattctgcag acaaatggca gtattcatcc acaattttaa
       61 aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac
      121 agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta
      181 ttacagggac agcagagatc cagtttggtt agtaccgggc ccgctctaga ttgaattatc
      241 acaactttgt atagaaaagt tggccctgca ggcgttacat aacttacggt aaatggcccg
      301 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
      361 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
      421 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
      481 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
      541 cagtacatct
//
